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Showing: 1 - 14 of 14 results
  1. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...blocked by alpha2-AR antagonist, nLight sensors are not. Instead, they are blocked by alpha1-AR antagonist. .... This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine release... in experiments, including ones that study alpha2-AR. Selectivity Norepinephrine and dopamine are similar...
  2. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...vectors contain an Autonomously Replicating Sequence (ARS) derived from the yeast chromosome. As the name suggests...considered low copy vectors and incorporate part of an ARS along with part of a centromere sequence (CEN). These...thus typically found as a single copy. Unlike the ARS vectors, CEN vectors are stable without integration...vectors. S. pombe plasmids oftentimes utilize an ARS to aid in high transformation efficiency; however...
  3. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ... Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA (2017) A Broad-Spectrum Inhibitor...Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016) Naturally Occurring Off-Switches for CRISPR-Cas9...
  4. Qi Lab CRISPR Page

    Type
    Collection
    ...endogenous CXCR4 gene 46920 pTDH3-dCas9 Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled...by TDH3 promoter 46921 pTDH3-dCas9-Mxi1 Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to N 51025...into HEK293 genome 46922 pSNR52-sgTEF1 Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR...endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR...
  5. Cre-lox system

    Type
    Collection
    ...pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding domain CMV Mammalian Green 12496 p270 pCMV-CRE-M-AR(LNCaP...fused to mutant AR ligand binding domain CMV Mammalian Green 12497 p274 pCMV-CRE-M-AR(T) CREM fused to...to mutant AR ligand binding domain CMV Mammalian Green 12529 pBS MCK CRE Muscle-specific expression of...
  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for CRISPR-Cas inhibition...26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013. Bacteriophage genes that inactivate the CRISPR...Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-switches for CRISPR-Cas9...
  7. CRISPR Guide

    Type
    Guide
    ... Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA. Cell . Sep 7;170(6):1224-...Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. Cell . 167(7):1829-1838. PMID: 27984730 Practical...Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. Nature . 526(7571):136-9. PMID: 26416740 Multiplexable...2013. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. Nature . 493(7432):429-32. PMID: 23242138 CAS9 transcriptional...
  8. CRISPR Guide

    Type
    Collection
    ... Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA. Cell . Sep 7;170(6):1224-...Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. Cell . 167(7):1829-1838. PMID: 27984730 Practical...Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. Nature . 526(7571):136-9. PMID: 26416740 Multiplexable...2013. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. Nature . 493(7432):429-32. PMID: 23242138 CAS9 transcriptional...
  9. Immunology Research Plasmids and Resources

    Type
    Collection
    ...apelin receptor AGTRL1, APJ, APJR, FLJ90771, MGC45246 AR androgen receptor AIS, DHTR, HUMARA, HYSP1, KD, NR3C4... NR3C4, SBMA, SMAX1, TFM AREG amphiregulin AR, CRDGF, MGC13647, SDGF ARMET arginine-rich, mutated in early...SLURP1 secreted LY6/PLAUR domain containing 1 ANUP, ARS, ArsB, LY6LS, MDM SORT1 sortilin 1 Gp95, NT3 SPP1...
Showing: 1 - 14 of 14 results