Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
Type
Blog Post
... acoustic gene 1 (arg1) and acoustic gene 2 (arg2). The key difference between arg1 and arg2 vesicles ... arg2 vesicles collapse at a lower pressure than arg1 vesicles. Following collapse, vesicles are invisible... ultrasound, which allows for differentiation of arg1 and arg2 ultrasound signals. This also means that...enough amplitudes to first collapse arg2 and then arg1 vesicles. By comparing ultrasound images pre-collapse...pre-collapse and post-collapse of arg2 and then arg1, each ARG’s distinct ultrasound fingerprint can be detected...growth following acoustic collapse E. coli re-express arg1 gas vesicles and restore their ultrasound signal...application for ARG’s: in vivo imaging. Specifically, arg1 expressing E. coli aided in ultrasound imaging of...