...interest (blue) in a plasmid backbone. A separate plasmid contains the transposase gene (red) for expressing....12137. PubMed. Find the Plasmids at Addgene Kowarz Lab plasmids Izsvak Lab plasmids  ...expressing the transposase enzyme.2.) The transposase enzyme is expressed (red stars) and binds the inverted...). The original plasmid is empty following the removal of the transposon; the plasmid is then degraded...insertion site.   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is one...translocate within the genome. The Sleeping Beauty transposase gains its name from the fact that it was ...was recreated from a defective transposase found in fish. This transposase has been discovered in the genomes...

...research tools: Sleeping beauty, PiggyBac, and Tol2.  Sleeping beauty Sleeping Beauty is a synthetic transposable...move antibiotic resistance genes between plasmids or from plasmids to the bacteria’s genome. In humans, if...the SB transposase, SB100X, is ~100-fold more efficient compared to first generation SB transposase (Mátés...transposition activity (Voigt et al., 2016). Sleeping Beauty plasmids  piggyBac Although its name suggests otherwise...codon-optimized wild-type piggyBac transposase.  piggyBac plasmids    Tol2  Tol2 was the first reported...module with the crRNA, 3) a transposase complex made up by three transposase proteins (TnsA, TnsB, TnsC...DNA site to another. This protein is either a transposase or a reverse transcriptase, depending on the ...

... 1998). Interested in plasmids that contain the groucho gene? Check out plasmids deposited by the Shivdasani... several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however was....   Sleeping Beauty, a transposon system from ancient fish Surprisingly, Sleeping Beauty is not only a...reconstructed a transposase system based on the naturally mutated and non-functional transposase gene found...was aptly named Sleeping Beauty (Ivics et al., 1997). We have a lot of plasmids that contain components... to see for yourself. We have one plasmid in our repository, plasmid 39624, that contains cmr.   tinman...website for plasmids that contain tinman or the mammalian homolog Nkx2.5. We also have one plasmid, Addgene...

... involving two-plasmid or single-plasmid systems (Vo et al., 2020). In the two-plasmid system, an effector...tools for human therapeutics (e.g. using Sleeping Beauty). Despite their diverse utility, DNA transposons...their limited programmability: depending on the transposases involved, they insert either randomly, or at...combining the DNA integration capabilities of transposases and the target programmability of CRISPR-Cas...Sternberg group, and CAST (CRISPR-associated Transposase) by the Zhang group. Mechanism of CRISPR-transposons...DNA-targeting module together with the crRNA.  Three transposase proteins (TnsA, TnsB, TnsC). The donor DNA (aka...RNA-DNA base pairing. Separately, TnsABC forms a transposase complex with the mini-transposon, excises it ...

...the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source...While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please read on to learn... components, a transposon and a transposase. The piggyBac™ transposase facilitates the integration of ...controlled by titrating the transposase to transposon ratios. High ratios of transposase to transposon result...piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor plasmid carrying...with the wild-type piggyBac™ transposase enzyme [4]. By using a donor plasmid containing a negative selection... that of the wildtype piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not...