Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 9 results for: blasticidin resistance

Showing: 1 - 9 of 9 results

  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ... Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, ...plasmid contains a cassette that will make cells resistant to a toxin), whereas negative selection would ...reagent). Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending ...confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin is typically...Backbone Read More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All... antibiotic resistance...

  2. Lentiviral Prep Service

    Type
    Collection
    ...Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human ...-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone..., N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (...transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A,...fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4)...

  3. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In their Nature...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent...

  4. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ..., or other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...metal ions, scientists have adapted an antibiotic resistance operon from E. coli : the tetracycline repressor... puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection. ...Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an insert...Blast (w756-1) Lentiviral Tet-On vector with blasticidin selection. See Plasmid #26730 for hygromycin ...selection. rtTA3 Eric Campeau 128061 pLVX-Tet3G blasticidin Lentiviral Tet-On vector with CMV promoter Tet-On...-On vector for CMV-driven rtTA with EGFP and Blasticidin selection rtTA-Advanced Mikhail Alexeyev 17492...

  7. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete: 10% v/v FBS...lentiCas9-Blast and then selected with 1 µg/mL blasticidin for 9 days. Single cells were then plated in...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more empty backbones on our Plant...Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA ...loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd...promoter for Agrobacterium-mediated transformation Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector ...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...226567 siRNA-resistant SigmaR1-mCherry SIGMAR1 mCherry CMV ALS Gia Voeltz 226569 siRNA-resistant pLV-SigmaR1...Hurley 237478 pLCKO-CMV-TMEM106B-P2A-Blasticidin TMEM106B P2A, Blasticidin CMV Frontotemporal dementia in GRN...pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin TMEM106B P2A, Blasticidin CMV Frontotemporal dementia in GRN...Pellman 238130 pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human TMEM106B P2A, Blasticidin, HA CMV Frontotemporal...Daelemans 238133 pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse TMEM106B P2A, Blasticidin, HA CMV Frontotemporal...SIGMAR1 mNeonGreen CMV ALS Gia Voeltz 226571 siRNA-resistant SigmaR1-mNeonGreen F191A SIGMAR1 mNeonGreen CMV...CMV ALS Gia Voeltz 226572 siRNA-resistant SigmaR1-mNeonGreen R119A SIGMAR1 mNeonGreen CMV ALS Gia Voeltz...
Showing: 1 - 9 of 9 results