...Function Antibiotic Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human ...-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. Lentiviral backbone..., N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (...transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A,...fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446 pLenti CMV GFP Hygro (656-4)...

... Conferring Resistance Cell Types* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, ...plasmid contains a cassette that will make cells resistant to a toxin), whereas negative selection would ...reagent). Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending ...confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin is typically...Backbone Read More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All... antibiotic resistance...

...Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434 pMA2640 Retroviral; CMV-...underlying expression of Tn10 encoded tetracycline resistance. Hillen W and Berens C. Annu Rev Microbiol . ...replaced with tTA, and Puromycin was replaced with Blasticidin selection. Please NOTE, this vector contains ...(rtTA3) expression vector, CMV promoter, and Blasticidin; can be used to make cell lines Tet-On Advanced...CMV-driven; linked via IRES to EGFP-Blasticidin fusion; pMA2641 has rtTA driven by retroviral LTR promoters...

...determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In their Nature...16 variants with different selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent...

..., or other. Bacterial Resistance Enter bacterial antibiotic resistance encoded in the plasmid that...glycerol stock. Choose from: ampicillin, apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin...

... lentiviral backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck...

...vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation DMEM Complete: 10% v/v FBS...lentiCas9-Blast and then selected with 1 µg/mL blasticidin for 9 days. Single cells were then plated in...