We narrowed to 3 results for: control scrambled shrna

... that contains a scrambled shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi...expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini’s lab is...RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the ...transfer plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene...particles. It’s capable of inducible expression of shRNA in target cells by the addition of tetracycline/...

...reporter M50 Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as...item of 2005. shRNA - In 2006, we saw an increase in popularity of empty backbones for shRNA expression,...

....1 – TRC control Negative control vector containing non-hairpin insert. pLKO.1 – scramble shRNA Negative...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles....Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal 21-mer... Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2...which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse ...genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community...lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct...