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We narrowed to 7 results for: control shrna vector plko 1 puro

Showing: 1 - 7 of 7 results
  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab...contains a scrambled shRNA. This scrambled shRNA is used as a negative control for mammalian RNAi experiments...provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes...and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: ...pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses...scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd generation lentiviral vector that contains...
  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...empty backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral...Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we...Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. ...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...
  3. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post
    ...Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest pLenti-puro (Plasmid...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning... vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Additionally, retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever expanding...
  4. Lentivirus Plasmids

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    Collection
    ... Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid...Diane Mathis 21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. See plasmid...selection. Bob Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid. Includes a ...engineered into viral vectors for safe use in the lab. One safety feature of lentiviral vectors is that the components...stuffer for easy cloning. David Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker. See plasmid...(Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See Ventura et al. ... 12247 pLVTHM 2nd EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter. Didier...
  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression pLJM1-EGFP... 3rd gen lentiviral vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible...selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro - ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...Reporter pGL3 Basic Vector - Firefly luciferase vector for investigating regions controlling transcription ...basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors for cloning in shRNAs, including plasmids...lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral...
  6. Tetracycline Inducible Expression

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    Collection
    ...21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA with puromycin selection...85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design of inducible shRNA expression... pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate shRNA efficacy by fluorescence, use Plasmid...choose between: Dual vector system: express the transactivator and the tet-controlled gene of interest from...separate vectors. Stable cell lines or transgenic animals can be made to achieve greater control over expression...transactivator and the tet-controlled gene of interest from one plasmid. A single vector approach may not have...have as much flexibility and control as a dual vector design, but it is easier to implement and ensures...
  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... the pLKO.1 TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector...Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone...non-hairpin insert. pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the... Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer ...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...
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