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We narrowed to 89 results for: cre recombinase

Showing: 1 - 20 of 89 results

  1. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce recombination...expression of Cre from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor... using multiple recombinases. To make multiple, precise genome edits, the recombinases used must have ...stop sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...however, the other recombinase/reporter pairs were inactive, even at very high recombinase levels. These data...toxicity of these recombinases by crossing flies homozygous for a UAS-driven recombinase with flies heterozygous...

  2. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...under the control of Flp recombinase together with inducible Cre recombinase (CreERT2), visualizes individual...the control of Cre recombinase are used in combination with mouse lines that express Cre only in the cell...Katrin Michel. Cre-lox is an incredibly popular and powerful site specific recombinase (SSR) system, but...LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the...control without modification - either Cre is there or it’s not. Cre-mediated possibilities for site specific...control FLP and Cre expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how... works in a similar fashion to the Cre system. The FLP recombinase recognizes FRT sites and initiates ...

  3. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 ...AAV5 Recombinases Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen...Collection. These preps drive expression of either Cre recombinase or a fluorescent marker in the various cell...- pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) AAV PHP.eB Recombinases Jonathan Ting New viral...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) AAV PHP.eB Recombinases Jonathan Ting New viral...- pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) AAV PHP.eB Recombinases Jonathan Ting New viral...

  4. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...expression of other recombinases. Multiple orthogonal attB-attP pairs per recombinase may be arranged on...different recombinases are overlapping or nested on a register, then the operation of one recombinase on the...they can control recombinase activity. For example, post-transcriptional recombinase activation via RNA...reduces the content of our paper, “Synthetic recombinase-based state machines in living cells” (1), into...explanation of how to adapt our engineered devices, recombinase-based state machines for your own experimental... about the order or timing of these factors. Recombinase-based state machines (RSMs), which take on a ...drives expression of a distinct large serine recombinase that recognizes its own attB and attP recognition...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...have created a cassette that will express GeneR only in the presence of Cre. In this example, Cre would...orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA fragment...orientation specificity of the site-specific recombinases (SSRs) Cre and FLP. SSRs bind DNA at target sites ...induce site specific recombination events: Cre recombinase binds loxP sites, while FLP binds FRT sites...and a pair of FRT sites and include both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen... absence of, or mCherry in the presence of Cre recombinase.   Once the SSR of choice is introduced...events are impossible even in the presence of Cre recombinase. This plasmid now specifically drives expression...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox system is a technology ...marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing Cre downstream...by making the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be expressed...constructed. Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully...components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. The P1 bacteriophage...components for use in genome manipulation. Cre recombinase, originally named because it “causes recombination...How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event...

  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors...design Recombinase-dependent vectors utilize pairs of specific sequences, lox (recognized by Cre) or frt...this spurious expression?   Figure 1.  Cre-recombinase binds to either pair of lox sites, let's say... On the left homologous pairs of scrambled cre-recombinase sites lead to greater frequency of reversion...system is off-target or spurious expression of recombinase-dependent AAV vectors. In this article, we will...excise a gene that is located between them. Early recombinase-dependent vector designs used a lox-STOP-lox ... to prevent transcription in cells where the recombinase was not present (Kuhlman, SK. 2008). While theoretically...

  8. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ... as a Cre-dependent Flp-expressing AAV. See our recombinase AAV inventory here New recombinases Plasmid... EGFP Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional control... µL size serotype testing) pAAV-hSyn-EGFP Recombinases Plasmid Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...inventory of recombinases and recombinase-dependent vectors. We recently released several recombinases in various...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...expression. This is a Cre-dependent switch that expresses nuclear mCherry in the absence of Cre, and expresses...Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments...expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors and GABA sensors) Calcium sensors...sensor (see below), in addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV Targeting and manipulating specific, genetically-defined...

  10. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...C-terminal and N-terminal Cre fragments together and permitting recombinase activity. Opto-Cre-Vvd is precise, ... inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases are enzymes...RFP reporter plasmid for recombinase activity were exposed to blue light. Cre-Vvd showed substantially...Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions...light. Unlike other split-Cre systems which can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut...pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8, as well as AAV5 and AAVrg. Find the Cre-expressing...improved activity as compared to other recombinase/photodimer pairs. Created with BioRender.com In their ...

  11. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as Cre-lox allow for more accurate ...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and ...coexpressed. Three new recombinases from the Cepko Lab are now available as AAV1. CRE-DOG constructs 69570...dependent Cre. The two segments come together in the presence of GFP to form a functional Cre enzyme that...ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-...control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression in forebrain...future therapies. Our popular plasmid encoding a Cre dependent version of the DREADD hM3D is now also ...

  12. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ....GFP-Cre.WPRE.SV40 AAVrg Recombinase James M. Wilson New serotype pENN.AAV.CamKII 0.4.Cre.SV40 AAVrg...pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase...James M. Wilson New serotype pENN.AAV.hSyn.Cre.WPRE.hGH AAV2 Recombinase James M. Wilson New serotype ...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) AAV-PHPeB Recombinase Jonathan Ting New viral...AAVrg Recombinase James M. Wilson New serotype AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias:...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...However, the Cre-dependent AAV cargo would only become activated in the presence of Cre recombinase, which ...by injecting a Cre-dependent AAV into Broca’s area of a mouse specifically expressing Cre in the hippocampus...phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection...genetically controlled function. By using AAVrg in Cre transgenic mouse lines, you can access and manipulate...hippocampus (Fig. 3). The Cre-dependent AAV would access the neurons (Fig. 3A) that project to Broca’s area...titers for efficient retrograde transport. For recombinase-dependent vectors, this can cause challenges ...projection pathway neurons and then inject a recombinase-dependent chemogenetic vector directly into the region...

  14. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Dre Plasmids...your favorite site-specific recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort...expressing Cre and other site-specific recombinases. Many of our in-stock viral preps feature Cre-dependent...Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome...Plasmids Flp Plasmids Other Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled...into two distinct families, serine recombinases and tyrosine recombinases, based on sequence similarity and...best characterized SSR systems, Cre and Flp, are tyrosine recombinases derived from P1 E. coli bacteriophage...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... in recombinase-dependent sequences. Recombinase-dependent sequences may be subject to recombinase-independent...pre-recombined population that does not require the recombinase for transgene expression. Once packaged in AAV...pre-recombined DNA could lead to transgene expression in recombinase-negative animals, producing misleading results... hundreds of viral vectors and estimate that recombinase-independent recombination occurs in 0.1-0.6% ...transgene expression but limits the likelihood of recombinase-independent expression. Next-generation sequencing...parallel because the risk of accidental errors increases. In addition to human error, fragments of DNA ...to the reference sequence of the plasmid used to create the viral prep. In a clean viral prep, like AAV...

  16. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...Although Cre isn’t a modular protein, Tang et. al were able to create a split version of Cre that is only...Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP ...started with another biological workhorse, Cre recombinase, which induces recombination at LoxP sites.... of interest blocks transcription when Cre is absent. When Cre is present, it removes the STOP cassette...when dimerized by the GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives... cord. Cre-DOG is also suitable for optogenetic studies, and since cells infected with AAV-Cre-DOG retain...

  17. Retrograde AAV viral preps

    Type
    Collection
    ...AAV-pgk-Cre PGK Cre expression Recombinases Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre and physically-separate... 0.4.Cre.SV40 CamKII Cre expression Recombinases Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre expression...mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637...pEF1a-DIO-FLPo-WPRE-hGHpA EF1a Cre-dependent Flpo recombinase Recombinases Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent...expression Recombinases Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases Wilson 105558... expression Recombinases Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases Wilson 55634...Flp-dependent Cre recombinase Recombinases Engel , Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression ...

  18. Deisseroth INTRSECT Collection

    Type
    Collection
    ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff EYFP Cre AND...AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT.../Von-EYFP Cre AND Flp AND Vcre Yes 137165 pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre No References...plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression of...approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression of...intersectional cell population definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...site-specific serine recombinase. First, the prime editor installs the recombinase “landing sites,” then...then the recombinase handles the installation of the donor sequence. The latest work evolves and engineers...engineers Bxb1 recombinase variants to optimize PASSIGE efficiency (Pandey et al., 2024). The best-performing...editing installs two sequences containing attB/P recombinase landing sites on opposite DNA strands. When the...edited sequence with the attB/P landing site. A recombinase integrates a sequence from a donor plasmid containing... in mammalian cells via continuously evolved recombinases and prime editing. Nature Biomedical Engineering...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...

  20. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...limiting amount of AAV are injected into the brain of Cre-expressing mice to trigger recombination and expression... 2019. Sparse Predictive Activity through Recombinase Competition (SPARC), a genetic tool that allows...expressed and the cells are not labeled. PhiC31, a recombinase. It specifically integrates an attP site into...variety of fly cell types. Because the PhiC31 recombinase can work in other model organisms including mouse...site upstream of the stop cassette results in decreased efficiency of recombination and preferentially...
Showing: 1 - 20 of 89 results