Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 3 results for: dcas9 krab bfp

Clear keywords
Showing: 1 - 3 of 3 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for...pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9-D3A) constructs. For lentiviral expression, Fuw-dCas9-Dnmt3a...Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a small DNA methyltransferase...Fusing dCas9 to a transcriptional activator like VP64 or VPR activates transcription, whereas dCas9-KRAB...activators/repressors - dCas9-p300 increased transcriptional activation more than dCas9-VP64, especially when...Dnmt3a and Fuw-dCas9-Dnmt3a-P2A-tagBFP are available from Rudolf Jaenisch’s lab, with the former also available...Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of epigenetic modifiers to specific...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G Tet-inducible expression of KRAB-dCas9-DHFR for CRISPRi...Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible expression of SP-dCas9-VPR transcriptional activator...Tet-On 3G rtTA TRE3GS Jason Sheltzer 11651 pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression...2006 ) for Tet-Off and additional plasmids. tetR-KRAB TRE Patrick Aebischer , Didier Trono 41393 pCW57.1...monosynaptic tracing. To be coinjected with pAAV-TREtight-mTagBFP2-B19G ( Plasmid #100799 ). Ian Wickersham 172878...

  3. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT] TARDBP CMV ALS Rajat Rohatgi 107840 pHBS1108 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 ∆CTD] TARDBP CMV ALS Rajat Rohatgi 107843 pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xsigma] TARDBP CMV ALS Rajat Rohatgi 107845 pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xsigma] TARDBP CMV ALS Rajat Rohatgi 107847 pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295S] TARDBP CMV ALS Rajat Rohatgi 107849 pHBS1337 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348S] TARDBP CMV ALS Rajat Rohatgi 107851 pHBS1285 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G348V] TARDBP CMV ALS Rajat Rohatgi 107854 pHBS1331 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43...
Showing: 1 - 3 of 3 results