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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5 results for: dcas9 vp64 blast

Showing: 1 - 5 of 5 results

  1. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP...with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) Zhang Pooled Barcoding Libraries... dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with ...Resistance Activity PI 52962 lentiCas9-Blast Sp Cas9 Cut Blasticidin Expresses human codon-optimized S. pyogenes...56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 human genes...Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library in backbone...non-targeting controls. This backbone does not contain dCas9. 57,050 unique sgRNAs targeting 18,901 human genes...

  2. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...dead Cas9 (dCas9) based activators (such as dCas9-VP64) or repressors (dCas9-KRAB or dCas9-SRDX) can also... W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ...production : 61425 : lentiviral vector encoding dCAS9-VP64 with 2A Blast resistance marker 61426 : lentiviral vector...for transient transfection : 61422 : Expresses dCAS9-VP64 activator with 2A GFP 61423 : Expresses the MS2...and SpCas9 together 2 vector system - lentiCas9-Blast and lentiGuide-Puro (sgRNA) Browse the Addgene GeCKO...three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2 RNA aptamers...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...target genes (20-40x greater than standard dCas9-VP64 activators) when introduced into mammalian cells....creating a new dCas9-VPR expression plasmid that uses the piggybac transposase to integrate dCas9-VPR into ... is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...methylation by fusing catalytically inactive Cas9 (dCas9) with the catalytic domain from DNA methyltransferase...Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3, pTALYM4 ... activator developed by the Church lab, termed “dCas9-VPR”, which facilitates robust activation of target...activate target genes, the relatively large size of dCas9-VPR limits one’s ability to package it into lentivirus...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian Lentiviral expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent...separate accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Mammalian/Lentiviral See paper none S. pyogenes Blast Sabatini and Lander lentiCRISPR v2 52961 Mammalian...Mammalian U6 yes, nick S. pyogenes Conklin pXFokI-dCas9 60901 Mammalian U6 yes, FokI S. pyogenes Conklin... 68709 Other/Trypanosoma cruzi none S. pyogenes Blast Docampo pDonor_hU6 69312 Mammalian hU6 none S. pyogenes...Mammalian U6 none S. aureus Joung pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 71236 Mammalian/Lentiviral hU6 yes...
Showing: 1 - 5 of 5 results