15 Years of Addgene: The Top 15 Plasmids
Type
Blog Post
...standard plasmids. Find pLKO.1 - TRC cloning vector.
Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...experiments. Find the scrambled shRNA vector.
pLKO.1 - TRC cloning vector - This vector from the David Root lab...provided by the Weiderschain lab. Find Tet-pLKO-puro.
lentiGuide-Puro - This plasmid expresses the S. pyogenes...capable of inducible expression of shRNA in target cells by the addition of tetracycline/doxycycline to the... pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors and an envelope...pSpCas9(BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses...scramble shRNA - This plasmid from David Sabatini’s lab is a 3rd generation lentiviral vector that contains...