We narrowed to 17 results for: flpo
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TypeBlog Post...Serotype Name 55637 AAVrg pAAV-EF1a-Flpo 87306 AAV1 AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors...55634 AAV1 pAAV-EF1a-mCherry-IRES-Flpo 55637 AAV1 pAAV-EF1a-Flpo Chemogenetics Plasmid Serotype...
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X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post...gene pXPR_068 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 L-CHIME Knocks out a single...sequentially pXPR_220 Violet-excited GFP (vex) Rosa26-FlpO-ERT2; H11-Cas9 Table 1: X-CHIME systems Proof... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...55634 AAV1 pAAV-EF1a-mCherry-IRES-Flpo 55637 AAV1 pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1) Viral vectors coming... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...targeted expression DREADDs GFP dependent Cre and FLPo And more! New lentiviral preps SARS-CoV-2 protein...cells expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. ... -
Hot Plasmids - August 2020
TypeBlog Post...New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available... -
Recombinases AAV Preps
TypeCollection...5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA Syn none...Zeng 174378 pAAV-syn-Flpo Syn none rg* Wickersham 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a...Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none... Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse...pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible Recombinases... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...recombinase. FLPe codon optimization resulted in FLPO, which shows similar efficiency to Cre (Kranz et...An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20. PubMed PMID... -
Retrograde AAV viral preps
TypeCollection...AAV phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Zeng 174378 pAAV-syn-Flpo Syn Flpo expression ...Recombinases Wilson 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a Flpo and physically-separate mCherry expression ...expression Recombinases Deisseroth 55637 pAAV-EF1a-Flpo EF1a Flpo expression Recombinases Deisseroth 55638 pAAV-EF1a-vCre...Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a Cre-dependent Flpo recombinase Recombinases Zhang ...expression Recombinases Wickersham 183412 pAAV-CAG-FlpO CAG Flpo expression Recombinases Janelia 51085 AAV-hSyn1... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...Isocortex 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Layer 6_IT_Car3 Isocortex 224000...Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex...Isocortex 230607 pAAV-AiE2103m-minBG-FlpO-WPRE-HGHpA AiP1252 AiE2103m FlpO Lamp5 interneurons Isocortex 223971...subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720...214855 pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1...Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA AiP14573 AiE0452h FlpO DRD2 Medium spiny neurons (D2-MSN...3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA AiP12040 AiE0354m FlpO Vip interneurons Isocortex 230101 pAAV-hsA2-AiE0219h-minRho-SYFP2... -
New Viral Vectors - Winter 2025
TypeBlog Post...Optogenetics Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep ... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post... Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA Additional resources on the Addgene... -
Cre-lox system
TypeCollection...129419 pCag FlpO-2A-Cre EV FlpO-2A-Cre CAG Mammalian Breunig 130986 pCag SE (Self-excising) FlpO-2A-Cre EV...pCAG-Cre Cre CAG Mammalian Chenn 26745 pDIRE iCre and FlpO, required for dual RMCE EF-1 alpha Mammalian Zeller... EV Self-excising FlpO-2A-Cre CAG Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible...translocation depending on the orientation of the FRT sites. FlpO constructs Addgene Blog Advanced Uses of Cre-lox... -
Deisseroth INTRSECT Collection
TypeCollection...pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo None Yes 55634 pAAV-EF1a-mCherry-IRES-Flpo None Yes 55638 pAAV-EF1a-vCre...pAAV-EF1a-mCherry-IRES-Dre None No 183535 pAAV-CaMKIIa-Flpo None No Single recombinase-dependent Addgene ID ... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...ssDNA. Here, we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018)... -
AAVED
TypeCollection...Breakfast and Welcome Melina Fan 9:30 AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing ... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection.... pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian/AAV none S...