Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
Type
Blog Post
...cells cotransfected with GFP and the GBPs, GFP linked the two GBPs together to create a complete transcription... al. tested pairs of their GFP-binding proteins (GBPs) to find those that could co-occupy GFP. Once they...of Cre that is only active when dimerized by the GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP...they constructed three components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding domain); and a ...