CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
Type
Blog Post
...selecting a sgRNA
Addgene may carry a validated sgRNA for your target gene. Validated gRNAs are a great...new sgRNA for your experiment, select from a variety of empty sgRNA vectors and design your sgRNA targeting...other.
If having two sgRNAs isn’t feasible for your experiment, ensure your sgRNA sequence has little ...of the many freely available sgRNA design programs.
Find Validated gRNAs for Your Next CRISPR Experiment...serve as the target for Cas9’s single guide RNA (sgRNA), provided it meets two conditions:
1) The sequence...off-target effects are a worry, consider a dual-sgRNA system. This system uses a “nicking” Cas9, in which...In order to produce a double stranded break, two sgRNAs must be used to target two Cas9-nickases (Cas9n...