22 Hot Plasmid Technologies from 2014
Type
Blog Post
...1143-8.
A new tool for CRISPR gRNA validation
Selecting a gRNA sequence that effectively targets...DNA break. Next, the gRNA being tested is expressed along with Cas9. If the gRNA effectively cuts the ...genome-wide knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids...plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means for large scale functional...for scientists to validate the efficacy of their gRNAs.
The first step is to clone the target sequence ...