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Showing: 121 - 140 of 239 results

  2. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a frameshift mutation... to a dead Cas9. The system is led, via a custom gRNA, to your gene of interest to temporarily increase...

  3. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...many gRNAs delivered with a relevant Cas nuclease. MoClo can be used to assemble multiplexed gRNA systems...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... system uses light to deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool...cerevisiae genes with 6-12 guides per gene where gRNA expression is induced by anhydrous tetracycline....

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...those generated by multiplexed delivery of multiple sgRNAs. Since multiplexing is commonly used to create ...helps the researcher see which of the multiplexed sgRNA was involved in a particular edit, and which type...

  11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in second a second...small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all of an AAV’s ~4.5 kB packaging...

  13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...In a subsequent study, they used CRISPR with two gRNAs to delete exon 51 or exons 45-55 in patient myoblasts...CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...

  14. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...or Cas fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...CRISPR Plasmids for Base Editing Find Validated gRNAs ...

  15. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ... day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing efficiency. On the third...

  18. Editor's Choice, August 2016

    Type
    Blog Post
    ...the transitions at target sequences specified by gRNAs. Caveats, potential specificity issues, and other...
Showing: 121 - 140 of 239 results