...: 38036853. Preprint: https://doi.org/10.21203/rs.3.rs-2941917/v1.  Ivorra-Molla, E., et al. (2023). A...: 38081970. Preprint: https://doi.org/10.21203/rs.3.rs-2684100/v1.  Piatkevich, K., et al. (2023). Bright...ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes...FIQ or mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to... of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with slightly...purify or image a recombinant protein, the FLAG tag has proven itself a good option for essentially all applications...western blots, though the original parent clone (M2) has been widely used in a variety of applications. Find...

...its cognate aminoacyl-tRNA-synthetase (aaRS) that has been evolved to incorporate non-canonical amino acids...aminoacylate the orthogonal tRNA. And the orthogonal tRNA has to bind to an unallocated codon. Therefore many controls... M. mazei Bacterial TAG Lital Alfonta 182881 Mut1-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine...propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram 182882 Mut2-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine...) Bacterial TAG Thomas Huber 177310 pRSF-G1(7FTrp)RS PylRS M. archaeon 7-Fluoro-L-Tryptophan (7FTrp) Bacterial...Bacterial TAG Ryan Mehl 141173 pAcBac1-3nitroY-A7-RS A7 3nY tRNA synthetase M. barkeri Mammalian Ryan ....1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA Y306A/Y384F (AF) pyrrolysine (Pyl) tRNA synthetase...

... tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus a successful base...luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris RS (2019) A panel of eGFP reporters for single base ...Brown WL, Donati F, Munagala U, Conticello SG, Harris RS (2018) A fluorescent reporter for quantification ...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ... Overlapping  Overview: The overlapping strategy has a region of homology, typically ~400-1400 bp long...Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression when compared...split AAV method While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing...efficiency of transgene expression from split vectors has been inconsistent. A greater understanding of basic...Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio A (2015) Improved dual AAV vectors with...

...well if you’ve been following the Addgene blog, it has been repurposed for genome editing in eukaryotes,...editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (Zhang et al., 2016...than that, be nice to your microinjectionist, who has the strategically important job of delivering the...10.1016/j.isci.2018.10.030 Qin W, Kutny PM, Maser RS, Dion SL, Lamont JD, Zhang Y, Perry GA, Wang H (2016...https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD...

...It is fair to say that CRISPR has revolutionized molecular biology and has enabled functional studies in...enzyme named Dicer (Figure 1). The plant kingdom has expanded its repertoire of Dicer enzymes and these...knockout methods of the same gene. However, recent work has called this prevalent assumption into question. Specifically...multiple target sequences are concatenated. hpRNA has been extensively used in plant research and many ...constructs are available to the community. Each construct has unique features, such as inducible expression of ...Bezanilla M, Perroud P ‐F., Pan A, Klueh P, Quatrano RS (2005) An RNAi System in Physcomitrella patens with...

...high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence in the absence...When exposed to UV light Padron is inactivated. The rs-Cherries (8) are another group of reversible PA-FPs...mitochondrial fusion is blocked during the Bax activation phase of apoptosis." J Cell Biol 164.4 (2004): 493-499...