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We narrowed to 8 results for: his tagged ubiquitin expression vectors

Showing: 1 - 8 of 8 results

  1. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...-baToN, “sender” cells express GFP on the surface and “receiver” cells express an anti-GFP nanobody on...response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern and ...highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged CRISPR knock-in... well as additional useful tags, including 3xFLAG epitopes, TEV and BioTag, which can be used in protein... protein effectivity), and a fluorescent protein tag for visualization (Fig. 1b). While the probes target... nanobody cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells...endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin ligase complex. In the presence of auxin, this...

  2. Plasmids 101: Protein tags

    Type
    Blog Post
    ...affinity tag is the polyhistidine (His) tag. Small and unlikely to affect function, His-tagged proteins...usually using a Ni2+ column. Like other affinity tags, a His tag can be fused to either the N- or C-terminus...Combo and cleavage tags Frequently, a single tag is not enough. What if you need one tag to increase solubility... to a purification scheme, His tags are frequently combined with other tags for dual-labeling. The problem...to all tags, but rather a quick overview of why scientists use tags, with a few time-tested tags and fusion...Fluorescent Protein Guide Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers...length a polyhistidine tract does not greatly alter the size of the tag.   Table 1: Common protein tags ...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His,GST...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...Mammalian expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral...Epitope tag pKMyc - N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc...with N-terminal Myc tag and attB for Drosophila transgenesis His Epitope tag pEZYmyc-His - C-terminal ...cerevisiae cells His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression...plants Find FP tagging vectors in budding yeast pHT101-mCherry - Expresses mCherry tagged fusion proteins...

  4. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Yeast Expression Vectors Find Bacterial Two Hybrid Plasmids Read our Blog Post About Yeast Vectors Read...the HIS3 gene, which is required for histidine manufacture and therefore growth on his-deficient media. ...system, histidine-auxotrophic host strains are used to select for binding-dependent expression of the ...activation. Vary the expression levels of bait and prey proteins - overexpression can lead to false interactions...During this period, proteins were successfully expressed as fusions that retain their individual activities...see Figure 2a) (4). These fusions are then co-expressed in a yeast strain that also contains a reporter...bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI’s interact, this...

  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...large-scale expression screen that can be used to generate His-tagged human kinase constructs that express well...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic...

  6. Genetic Code Expansion

    Type
    Collection
    ...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta...DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta ...Alfonta 160089 pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial TAG Lital Alfonta 160377 pDule-Mb haloTyrRS...coli pBof Mammalian TAG Huiwang Ai 68292 SepOTSλ SepRS9 E. coli Sep Bacterial TAG Jesse Rinehart 71403...Bacterial TAG Dieter Söll 104069 pTECH-chAcK3RS(IPYE) AcK3RS Chimeric Nε-acetyl-L-lysine Bacterial TAG David...Bacterial TAG David Liu 104071 pTECH-MmAcK3RS(IPYE) AcK3RS M. mazei Nε-acetyl-L-lysine Bacterial TAG David...Bacterial TAG David Liu 104073 pTECH-MmPylRS(IPYE) PylRS M. mazei m-iodo-L-phenylalanine Bacterial TAG David...

  7. Cre-lox system

    Type
    Collection
    ...KASH-tagged EGFP, and sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 ...Mammalian Sato 123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial Liu...present. By expressing Cre at specific times or locations, you can precisely control expression of your gene...developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter... or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the...visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher levels...

  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System... into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF from the...finds and attacks pathogens or infected cells expressing that antigen. Antibodies produced by B cells ...factor (TNF) family of proteins are predominantly expressed by immune cells and help to regulate the immune...regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory factor X-associated ankyrin-containing...associated protein C20orf188, TRRP4AP, TRUSS UBR1 ubiquitin protein ligase E3 component n-recognin 1 JBS, ...
Showing: 1 - 8 of 8 results