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Showing: 21 - 40 of 217 results
  1. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...delivery in mice. J Clin Invest 118:1955–1964 . https://doi.org/10.1172/jci34316 Bak RO, Porteus MH (2017...Using AAV Donor Vectors. Cell Reports 20:750–756 . https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho...Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503  Chamberlain ...Virus Capsids. Human Gene Therapy Methods 27:1–12 . https://doi.org/10.1089/hgtb.2015.140 Frischmeyer PA (...Lacking Termination Codons. Science 295:2258–2261 . https://doi.org/10.1126/science.1067338 Ghosh A, Yue Y...Transgene-independent Manner. Molecular Therapy 16:124–130 . https://doi.org/10.1038/sj.mt.6300322 Halbert CL, Allen...parts of a larger gene. Nat Biotechnol 20:697–701 . https://doi.org/10.1038/nbt0702-697 Hirsch ML, Agbandje-McKenna...
  2. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...epigenome editing. Nucleic Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito...and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199 Find plasmids from this...human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853 Find plasmids from this...-MQ1 fusion protein. Nature Communications 8: . https://doi.org/10.1038/ncomms16026 Find plasmids from...in the Mammalian Genome. Cell 167:233–247.e17 . https://doi.org/10.1016/j.cell.2016.08.056 Find plasmids...fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726 Find plasmids from this...site-specific DNA methylation. Biology Open 5:866–874 . https://doi.org/10.1242/bio.019067 Find plasmids from...
  3. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...targets. Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778 Erdmann, R. S., Baguley...SNAP-tags. Cell Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher...Reactivity. ACS Chemical Biology, 4(7), 547–556. https://doi.org/10.1021/cb900062k Gautier, A., Juillerat...Living Cells. Chemistry & Biology, 15(2), 128–136. https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin...Inside Live Cells. Science, 281(5374), 269–272. https://doi.org/10.1126/science.281.5374.269 Jing, C.,... Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f Keppler, A., Gendreizig...in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765 Lavis, L. (2016, September...
  4. A History of Genome Engineering in Popular Culture

    Type
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    ...double-strand breaks or donor DNA. Nature 576:149–157 . https://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod...The Journal of Experimental Medicine 79:137–158 . https://doi.org/10.1084/jem.79.2.137 Carroll D (2011) ...With Zinc-Finger Nucleases. Genetics 188:773–782 . https://doi.org/10.1534/genetics.111.131433 Doudna JA,...engineering with CRISPR-Cas9. Science 346:1258096 . https://doi.org/10.1126/science.1258096 Gaudelli NM, Komor...genomic DNA without DNA cleavage. Nature 559:E8–E8 . https://doi.org/10.1038/s41586-018-0070-x Griffith F (...Pneumococcal Types. Journal of Hygiene 27:113–159 . https://doi.org/10.1017/s0022172400031879 Hershey AD, ...BACTERIOPHAGE. The Journal of General Physiology 36:39–56 . https://doi.org/10.1085/jgp.36.1.39 Komor AC, Kim YB, ...
  5. CRISPR Activation: A Practical Guide

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    ...using CRISPR–Cas9 screens. Nature 568:511–516 . https://doi.org/10.1038/s41586-019-1103-9 Bialek JK, Dunay...Transcriptional Activator Systems. PLoS ONE 11:e0158294 . https://doi.org/10.1371/journal.pone.0158294 Chari R, ...library-on-library approach. Nat Methods 12:823–826 . https://doi.org/10.1038/nmeth.3473 Chavez A, Scheiman ...transcriptional programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M,...activators in multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Gilbert LA, Horlbeck...Gene Repression and Activation. Cell 159:647–661 . https://doi.org/10.1016/j.cell.2014.09.029 Konermann S...engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Pan J, Meyers RM, ...
  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...for codon usage tables. BMC Bioinformatics 18: . https://doi.org/10.1186/s12859-017-1793-7 Dresios J, Chappell...initiation in eukaryotes. Nat Struct Mol Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan...expression. Trends in Biotechnology 22:346–353 . https://doi.org/10.1016/j.tibtech.2004.04.006 Hausser ...inhibit translation. Genome Research 23:604–615 . https://doi.org/10.1101/gr.139758.112 Mauro VP, Chappell...therapeutics. Trends in Molecular Medicine 20:604–613  https://doi.org/10.1016/j.molmed.2014.09.003 Nakamura ... year 2000. Nucleic Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-...mutations to human disease. Nat Rev Genet 12:683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987...
  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...Clinical and Developmental Immunology 2012:1–15 . https://doi.org/10.1155/2012/980250  Bates A, Power CA...Prospects of Antibody Fragments. Antibodies 8:28 . https://doi.org/10.3390/antib8020028  Bird R, Hardman ...antigen-binding proteins. Science 242:423–426 . https://doi.org/10.1126/science.3140379  Bradbury ARM,...display technologies. Nat Biotechnol 29:245–254 . https://doi.org/10.1038/nbt.1791  Deal CE, Balazs AB (...infection. Current Opinion in HIV and AIDS 10:190–197 . https://doi.org/10.1097/coh.0000000000000145  Gąciarz ...intrinsically stable scFv. PLoS ONE 12:e0189964 . https://doi.org/10.1371/journal.pone.0189964  Huston JS...the National Academy of Sciences 85:5879–5883 . https://doi.org/10.1073/pnas.85.16.5879  Leavy O (2016...
  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...sequence-specific antimicrobials. Nat Biotechnol 32:1146–1150 . https://doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai...CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M...RNA-guided nucleases. Nat Biotechnol 32:1141–1145 . https://doi.org/10.1038/nbt.3011 Cobb RE, Wang Y, Zhao...Engineered CRISPR/Cas System. ACS Synth Biol 4:723–728 . https://doi.org/10.1021/sb500351f Dong C, Fontana J, Patel...transcriptional reprogramming in bacteria. Nat Commun 9: . https://doi.org/10.1038/s41467-018-04901-6 Jiang W, Bikard...CRISPR-Cas systems. Nat Biotechnol 31:233–239 . https://doi.org/10.1038/nbt.2508 Leonard SP, Perutka J...Broad-Host-Range Plasmids. ACS Synth Biol 7:1279–1290 . https://doi.org/10.1021/acssynbio.7b00399 Li Q, Sun B,...
  9. Viral Vectors 101: Optogenetic Tools

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    ...Photoswitching Proteins (Vol. 2173, pp. 21–51). Springer US. https://doi.org/10.1007/978-1-0716-0755-8_2 Lehtinen, ...Frontiers in Cellular Neuroscience, 15, 778900. https://doi.org/10.3389/fncel.2021.778900 Mattis, J., ...microbial opsins. Nature Methods, 9(2), 159–172. https://doi.org/10.1038/nmeth.1808 References Abreu, N...Photoswitching Proteins (Vol. 2173, pp. 21–51). Springer US. https://doi.org/10.1007/978-1-0716-0755-8_2 Boyden, E....activity. Nature Neuroscience, 8(9), 1263–1268. https://doi.org/10.1038/nn1525 Gunaydin, L. A., Yizhar...optogenetic control. Nature Neuroscience, 13(3), 387–392. https://doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y...triggering perception. Science, 365(6453), eaaw5202. https://doi.org/10.1126/science.aaw5202 Mattis, J., Tye...
  10. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...control of CRISPR–Cas9. Nat Methods 15:924–927 . https://doi.org/10.1038/s41592-018-0178-9  Hemphill J,...Cas9 Gene Editing. J Am Chem Soc 137:5642–5645 . https://doi.org/10.1021/ja512664v  Jain PK, Ramanan V,... Protectors. Angew Chem Int Ed 55:12440–12444 . https://doi.org/10.1002/anie.201606123  Kim JH, Rege M...gene expression control. Nat Methods 16:633–639 . https://doi.org/10.1038/s41592-019-0436-5 Mathony J, Hoffmann...of CRISPR‐Cas9. Angew Chem Int Ed 58:6285–6289 . https://doi.org/10.1002/anie.201900788 Nihongaki Y, Furuhata...neuronal differentiation. Nat Methods 14:963–966 . https://doi.org/10.1038/nmeth.4430 Nihongaki Y, Kawano...optogenetic genome editing. Nat Biotechnol 33:755–760 . https://doi.org/10.1038/nbt.3245 Nihongaki Y, Yamamoto...
  11. Viral Vectors 101: Pseudotyping

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    ...Protein for Neutralization Assays. Viruses 12:513 . https://doi.org/10.3390/v12050513 Cronin J, Zhang X-Y,...Lentiviral Vectors through Pseudotyping. CGT 5:387–398 . https://doi.org/10.2174/1566523054546224 DePolo NJ, Reed...Inactivated by Human Serum. Molecular Therapy 2:218–222 . https://doi.org/10.1006/mthe.2000.0116 Gutierrez-Guerrero...for Research and Gene Therapy. Viruses 12:1016 . https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval...Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089/hgtb.2017.084 Li Q, Liu Q, Huang...for enveloped viruses. Rev Med Virol 28:e1963 . https://doi.org/10.1002/rmv.1963 Mazarakis ND (2001) Rabies...delivery. Human Molecular Genetics 10:2109–2121 . https://doi.org/10.1093/hmg/10.19.2109 Sandrin V, Boson...
  12. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...methyl-CpG-binding protein 2. Nat Genet 23:185–188 . https://doi.org/10.1038/13810 Amir RE, Zoghbi HY (2000...phenotype-genotype correlations. Am J Med Genet 97:147–152 . https://doi.org/10.1002/1096-8628(200022)97:2<147::aid-ajmg6...Syndrome and Related Disorders. Cell 152:984–996 . https://doi.org/10.1016/j.cell.2013.01.038 Guy J, Gan ...Model of Rett Syndrome. Science 315:1143–1147 . https://doi.org/10.1126/science.1138389 Le TTH, Tran NT...Human-Induced Pluripotent Stem Cells. Front Genet 10: . https://doi.org/10.3389/fgene.2019.00625 McGraw CM, Samaco... Function Requires MeCP2. Science 333:186–186 . https://doi.org/10.1126/science.1206593 Powers S, Miranda...MeCP2 null (S51.002). Neurology 92 (15 Supplement) . http://n.neurology.org/content/92/15_Supplement/S51.002...
  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
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    ...targeting with CRISPR–Cas13. Nature 550:280–284 . https://doi.org/10.1038/nature24049 Find plasmids from...high-fidelity genome-editing platform. Genome Biol 19: . https://doi.org/10.1186/s13059-018-1591-1 Cox DBT, Gootenberg...editing with CRISPR-Cas13. Science 358:1019–1027 . https://doi.org/10.1126/science.aaq0180 Find plasmids...endonucleases in human cells. Nat Biotechnol 34:863–868 . https://doi.org/10.1038/nbt.3609 Find plasmids from this...derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038/ncomms14500 Find plasmids from...altered PAM specificities. Nature 523:481–485 . https://doi.org/10.1038/nature14592 Find plasmids from...nucleases in human cells. Nat Biotechnol 34:869–874 . https://doi.org/10.1038/nbt.3620 Find plasmids from this...
  14. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Find plasmids from...therapeutic application. Nat Biotechnol 38:892–900 . https://doi.org/10.1038/s41587-020-0491-6 Find plasmids...and cytosine editing. Nat Biotechnol 38:861–864 . https://doi.org/10.1038/s41587-020-0535-y Find plasmids... deaminase fusions. Nat Biotechnol 35:371–376 . https://doi.org/10.1038/nbt.3803 Find plasmids from this...ancestral reconstruction. Nat Biotechnol 36:843–846 . https://doi.org/10.1038/nbt.4172 Find plasmids from this...double-stranded DNA cleavage. Nature 533:420–424 . https://doi.org/10.1038/nature17946 Find plasmids from...efficiency and product purity. Sci Adv 3:eaao4774 . https://doi.org/10.1126/sciadv.aao4774 Find plasmids ...
  15. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...pAAV-EF1a-DIO-JEDI-2P-WPRE https://www.addgene.org/179460/ and pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE https://www.addgene.org...under CC-BY license.   Find MTRIA sensors here: https://www.addgene.org/browse/article/28225122/   Ino... Aggarwal 2022.   Find iGluSnFR3 plasmids here: https://www.addgene.org/browse/article/28220233/ Find ...here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt https://www.addgene.org/175180/    Aggarwal A, et al. ...CC-BY-NC license.   Find JEDI-2P plasmids here: https://www.addgene.org/browse/article/28223314/ Find ...under CC-BY license.   Find pYtags plasmids here: https://www.addgene.org/browse/article/28229067/   Farahani...AAV packaging.    Find iGECInano plasmids here: https://www.addgene.org/browse/article/28228652/   Matlashov...
  16. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Using CRISPR/Cas Systems. Science 339:819–823 . https://doi.org/10.1126/science.1231143 Doench JG, Hartenian...gene inactivation. Nat Biotechnol 32:1262–1267 . https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi...Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002/0471142905.hg1508s88 Leidy-Davis...CRISPR/Zygote Injection Approaches. Sci Rep 8: . https://doi.org/10.1038/s41598-018-33408-9 Mizuno N, Mizutani...Adeno-Associated Viral Vector. iScience 9:286–297 . https://doi.org/10.1016/j.isci.2018.10.030 Qin W, Kutny...Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002/9780470942390.mo150178 Qin W, ...Electroporation of Nuclease. Genetics 200:423–430 . https://doi.org/10.1534/genetics.115.176594 Yang H, Wang...
  17. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    ...methylated somatic enhancers. Nat Genet 52, 819–827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A...from their normal counterparts. Nature 301, 89–92. https://doi.org/10.1038/301089a0. Galonska C, Charlton...methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich...activity and function in development. eLife 7:e34870. https://doi.org/10.7554/eLife.34870.  Kaas GA, Zhong C... and Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita ...catalytic domain fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658. Sapozhnikov DM, Szyf...methyltransferase with CRISPR/dCas9. Nat Commun 12, 5711. https://doi.org/10.1038/s41467-021-25991-9. Spruijt CG...
  18. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ...enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554/elife.43322 Baker M (2015) Reproducibility...Blame it on the antibodies. Nature 521:274–276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012...preclinical cancer research. Nature 483:531–533 . https://doi.org/10.1038/483531a Bradbury A, Plückthun ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun...validate recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein... functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Crosnier...mouse monoclonal antibodies. BMC Biology 8:76 . https://doi.org/10.1186/1741-7007-8-76 Greenfield, E.A...
  19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Chavez A, Scheiman ...transcriptional programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M,...activators in multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham...engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak...transcription factors. Nat Methods 10:973–976 . https://doi.org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert...Control of Gene Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik...system differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-019-54179-x Tanenbaum ME...
  20. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...retroviruses. Microbiological Reviews, 56(3), 375–394. https://doi.org/10.1128/MR.56.3.375-394.1992. PubMed PMID...Current State and Perspectives. Viruses, 13(7), 1288. https://doi.org/10.3390/v13071288. PMID: 34372494. Prussin...Environmental Science & Technology Letters, 2(4), 84. https://doi.org/10.1021/ACS.ESTLETT.5B00050. PubMed PMID...of the United States of America, 54(1), 137–144. https://doi.org/10.1073/PNAS.54.1.137. PubMed PMID: 4285422...precursor protein. The EMBO Journal, 16(16), 5113–5122. https://doi.org/10.1093/EMBOJ/16.16.5113. PubMed PMID:...virus DNAs. Journal of Virology, 14(5), 1132–1141. https://doi.org/10.1128/JVI.14.5.1132-1141.1974. PubMed.... Journal of Molecular Biology, 79(1), 163–183. https://doi.org/10.1016/0022-2836(73)90277-5. PubMed PMID...
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