...gene therapy.” Journal of cellular physiology.  https://doi.org/10.1002/jcp.30064 2. Barman et al. 2020...with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294-019-01040-3 3. Khatibi ... Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610 4. Uddin...and Implications for the Future.” Front. Oncol. https://doi.org/10.3389/fonc.2020.01387 5. Rohn et al.... Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6. Wu et al....Therapy in Human Genetic Diseases.” Theranostics. https://doi.org/10.7150/thno.43360 7. Yuxuan Wu et al....

...Its Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier...Inherently Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui...Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu...Genome Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, ...during nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding...Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog...

... guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016/j.it.2012.02.010 Dennler P, Fischer...Populations to Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390/antib4030197  Hegazy M, Cohen-...Tissues in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org/10.1002/cpcb.115  Mueller BM, WrasidloO... to Monoclonal Antibodies. Hybridoma 7:453–456. https://doi.org/10.1089/hyb.1988.7.453  Niemeyer CM, Adler...quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267 Stoeckius M, Hafemeister...measurement in single cells. Nat Methods 14:865–868. https://doi.org/10.1038/nmeth.4380  Additional resources...

...protein–protein interactions in live Drosophila. eLife 7. https://doi.org/10.7554/elife.38853 Hu C-D, Chinenov Y...Fluorescence Complementation. Molecular Cell 9:789–798. https://doi.org/10.1016/s1097-2765(02)00496-3 Kerppola...interactions in living cells. Nat Protoc 1:1278–1286. https://doi.org/10.1038/nprot.2006.201 Kerppola TK (2013...Cells. Cold Spring Harb Protoc 2013:pdb.prot076497. https://doi.org/10.1101/pdb.prot076497 Kim Y, Jung JP,...Saccharomyces cerevisiae. Genome Res 29:135–145. https://doi.org/10.1101/gr.231860.117 Kodama Y, Hu C-D...year update and future perspectives. Biotech 53. https://doi.org/10.2144/000113943 Additional resources...

... DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Glaser A, McColl B...Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti A, Foronda ...activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango...APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin ...living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel...human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources...

...nucleobase detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP... detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, ...CRISPR nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico...on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N....Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh...detection of COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special...

...multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough...Immunofluorescence on Fresh Frozen Tissue-V2 v2. protocols.io. https://doi.org/10.17504/protocols.io.bs68nhhw Im, K.,...Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935-5_26 Jambor, H...scientific publications. PLOS Biology, 19(3), e3001161. https://doi.org/10.1371/journal.pbio.3001161 McLaughlin...Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke, A. J., Kandov...National Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 Additional Resources...

...protein analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette...protein A. Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori...the user, for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser...the National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin...the National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood...and trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources...

...plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath ...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang...editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner ... the National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger...translation efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources...

...Transgene Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. ... System. Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288....capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775...Adeno-Associated Virus. Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513....increases transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345...

...identifier format for lenti-Cas9-VQR is the URL “http://n2t.net/addgene:87155” where the Addgene registered... as “RRID:Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will...gift from Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” ...gift from Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite... gift from Daniel Bauer (Addgene plasmid # 87155 http://n2t.net/addgene:87155)” Cite plasmid using RRID...

...observational study. The Lancet Microbe 1:e14–e23 . https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB...Respiratory Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei ...cross-reactivity with SARS-CoV. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K,...model for SARS? Trends in Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open ...Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020-0499-y Additional resources...

... 2021. https://doi.org/10.7554/eLife.64631  Goertsen D, et al. Nature Neuroscience. 2022. https://doi....  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   dCas9-SSAP...plasmids here! Wang et al. Nat Cell Biol. 2022. https://doi.org/10.1038/s41556-021-00836-1 Visual barcodes...barcode plasmids here! Kaufman, et al. 2022. Nature Comm.https://doi.org/10.1038/s41467-022-30008-0       ...

...additional functional variable regions. mAbs 10:539–546. https://doi.org/10.1080/19420862.2018.1445456  Holzlöhner...Monoclonal Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman NS, Jackson LR, ...Information Resources. ILAR Journal 46:258–268. https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari...Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942  National Research...Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov/books/NBK100203/ Additional...

...primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi.org/10.1038/nbt.3290 Rozners, E. Chemical ... and Specificity. JACS 144, 12584-12594 (2022). https://doi/pdf/10.1021/jacs.2c02633 Allen, D., Rosenberg... Cells. Frontiers in Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu... gRNAs. Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016/j.molcel.2021.02.007 Moroz-Omori...CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https://pubs.acs.org/doi/10.1021/acscentsci.9b01093 More...

...factors modulating SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD...Protein for Neutralization Assays. Viruses 12:513. https://doi.org/10.3390/v12050513 Hsieh C-L, Goldsmith...prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501–1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam...recognition of evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna... Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 Additional resources...

...proto-oncogene product. Mol Cell Biol 5:3610–3616. https://doi.org/10.1128/mcb.5.12.3610-3616.1985 Munro ...domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984.tb02263.x  Munro...immunoglobulin heavy chain binding protein. Cell 46:291–300. https://doi.org/10.1016/0092-8674(86)90746-4 Munro S, ...secretion of luminal ER proteins. Cell 48:899–907. https://doi.org/10.1016/0092-8674(87)90086-9  Schüchner...on neighboring sequence context. Sci Signal 13. https://doi.org/10.1126/scisignal.aax9730  Additional ...

...in Molecular Biology. Humana Press, pp 133–148. https://doi.org/10.1007/978-1-62703-293-3_10 Walter J ...the SARS-CoV-2 receptor-binding domain. BioRxiv.  https://doi.org/10.1101/2020.04.16.045419.  Wrapp D, De...Single-Domain Camelid Antibodies. Cell 181:1004–1015.e15 . https://doi.org/10.1016/j.cell.2020.04.031 Zimmermann ...delicate proteins. Nature Protocols 15:1707–1741. https://doi.org/10.1038/s41596-020-0304-x Zimmermann I...conformational trapping of membrane proteins. eLife 7: . https://doi.org/10.7554/elife.34317 Additional resources...

.... Pubmed. Resources: http://openspim.org/Welcome_to_the_OpenSPIM_Wiki http://www.janelia.org/lab/keller-lab.../keller-lab http://www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html...ultramicroscope-overview.html http://www.zeiss.com/microscopy/en_de/products/imaging-systems/lightsheet-z-1.html ...

...Video on We Share Science: http://wesharescience.com/pin/7472 Article: http://www.cell.com/current-biology... on We Share Science: http://wesharescience.com/pin/7439 Research Website: https://umaine.edu/aquaculture...current-biology/abstract/S0960-9822(16)30873-9 Youtube: https://youtu.be/EHHhiawIdWM Saving salmon, one embryo ...