...with the sgRNA cassette on a plasmid expressing mCherry and resistance selection marker HygR. The CRISPR ...

...Their new method uses a selection cassette containing genes for hygromycin resistance and the Roller movement...heat-shock inducible Cre to generate a self-excising cassette (SEC). To make it possible fluorescently label...into the genome. Figure 1: Self-excising cassette schematic from Dickinson et al. (2015). Used with...Engineering with a Self-Excising Drug Selection Cassette. Dickinson DJ, Pani AM, Heppert JK, Higgins CD...young adult worms. Once the worms have laid eggs, hygromycin is added to the plates to select for candidate...

...including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG cassette.    Find gRNA multiplexing...smaller than constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ...monocot and dicot plants, with a choice of either hygromycin or Basta selection. Yang Lab Single Transcript...

...up positive traits (i.e. the plasmid contains a cassette that will make cells resistant to a toxin), whereas... a negative trait (i.e. the plasmid contains a cassette that will make cells sensitive to a toxin). In...80S; inhibits chain elongation 100-800 ug/mL Hygromycin B hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks...

... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) 71485 Mammalian none S. pyogenes Hygro Sherwood pMpGE_En01 71534 Other...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369...gRNA module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or ...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...

... to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus, and selection...destination vectors contain an ampicillin resistance cassette and an origin of replication, which are not schematized...Plasmid pRDB_052 (for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous...

...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure...recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly used for positive...

...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested.... SpCas9 alone: This plasmid contains a single cassette expressing human codon-optimized SpCas9; sgRNA...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...This plasmid ( PX600, #61592 ) contains a single cassette expressing human codon-optimized SaCas9; SaCas9...plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human codon-optimized SpCas9, driven...

...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs 11619...shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler 1730... plasmid with a chimeric 5’LTR, Any expression cassette (promoter and gene of interest) can be cloned ...interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). See...plasmid with puro resistance. See plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning ...expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression...expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for other versions...

...2) a transposon containing the gene-expression cassette that can translocate within the genome. The Sleeping...selection markers (blasticidin, neomycin, puromycin, hygromycin) and fluorescent protein reporters (GFP, BFP,...

...inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged...inducible expression of a miR-shRNA; selection cassette in format: rtTA3+IRES+Neo; see article for additional...conditional cell line generation; contains expression cassette for TetR-P2A-GFP; see article for additional constructs... rtTA On Alexeyev 14901 pCL-CTIG The inducible cassette contains tTA at its 5' end and an inducible promoter...make cell lines Tet-On Advanced; Plasmid 26730 has hygro resistance 3rd generation rtTA On Campeau 25434 ...

...p252 pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green 8408 p255 pPr-CREM/Ins...Ins/CMV-STOP-luc CREM and CMV-STOP-luc cassette, separated by insulator probasin Mammalian Green 11543...Sauer 12168 pMB80 (R26-CreER) Cre-ERT2 with loxp cassette; Targeting vector for Rosa26 locus Mammalian Jacks...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...activates gene of interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration...pPNT6 Cre activates gene of interest, removes Neo cassette. Used in gene targeting. Mouse Targeting Shivdasani...pMSCVpuro-Cre Cre 5'LTR Retroviral Ge 34565 pMSCVhygro-Cre Cre and HygroR coexpression Retroviral Ge 34568 WZLneo-Cre...

...create stable cell lines in which the expression cassette of interest is incorporated into the host...puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant... (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker Also see more empty backbones...Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG with rice snoRNA...

...backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus, allowing for Cre-dependent...donor vectors containing mAID and either Neo or Hygro resistance markers with CRISPR/Cas vectors targeting...