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Addgene
Showing: 61 - 80 of 222 results
  1. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...
  2. Antibodies 101: Conjugation

    Type
    Blog Post
    ...looking to conjugate antibodies yourself, there are kits available to make the process quick and easy. Before...antibody’s buffer is compatible with your conjugation kit, since many common antibody buffers and buffer additives...
  3. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... can be done in-lab using commercially available kits or ordered as synthesis modifications when purchasing...choose from and you can opt to do-it-yourself with a kit or purchase it commercially synthesized with a label...
  4. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA. This technique works by...interested in detecting in your sample. When an ELISA kit is ordered, the wells are typically already coated...
  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled...ligand-bound GPCRs of interest. The TRUPATH plasmid kit consists of 20 plasmids encoding 14 alpha, 2 beta...
  6. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of hours...day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted to...
  7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ... you prepaid shipping materials (i.e., a deposit kit) with instructions on how to send liquid DNA or bacterial...pages for your review. We’ll also mail you a deposit kit with instructions on how to prepare and ship the ...
  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol. DNA yields...lead to degradation of your prep. Many plasmid prep kits recommend performing an extra wash step to remove...
  9. Zebrafish Plasmid Collection

    Type
    Collection
    ... Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids...and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene...compartment-specific biotin labeling in zebrafish. pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based ...Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression...repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines from European...model potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary ...
  10. Cancer Research Plasmids and Resources

    Type
    Collection
    ...transcription factor and tumor suppressor. Wnt Coming soon! Kits and Libraries for Cancer Research Broad Target Accelerator...of a “pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing...Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer Institute...
  11. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple streams for composting...
  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent Protein Kits Browse the Fluorescent Protein... recently developed a modular plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging...offers for tagging a single genetic locus, the toolkit also offers repair templates for tagging proteins... elegans, several groups have designed modular toolkits to assist with tagging genomic loci in other model...obtained as a universal donor plasmid. The CRISPaint toolkit includes several donor plasmids for protein tagging... S2 cell line stably expressing Cas9. This PCR toolkit offers C- and N-terminal tagging vectors (eg GFP... Matthew L. and Eric M. Jorgensen. “SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification...
  13. TALEN Engineering

    Type
    Collection
    ... Genome Engineering TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering...are provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression...Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome editing...
  14. Viral Production at Addgene

    Type
    Blog Post
    ...mycoplasma contamination using mycoplasma detection kits. Cell line is maintained for ~20 passages before...prepared using endotoxin-free plasmid purification kits.     Figure 4: Schematic of the barcode ...
  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...Lentiviral Expression Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids...
  16. mTOR Pathway

    Type
    Collection
    ...Pathway ORF Kit Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening Kit Background...Pathway ORF Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening Kit (from David...
Showing: 61 - 80 of 222 results