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We narrowed to 123 results for: lenti crispr

Showing: 1 - 20 of 123 results

  1. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    Blog Post
    ... more about CRISPRs: Using CRISPR/Cas to Edit Disease Out of the Genome History of CRISPR Cas - A tale...system. Genome wide mouse CRISPR library The Yusa lab has leveraged the CRISPR system to target all 19,...genes all across the genome? Two new human lentiviral CRISPR library systems described in companion papers...article describes the development of a mouse lentiviral CRISPR library.  “This enables you to do customized...select gene groups The 6 available enriched lentiviral CRISPR libraries developed by MIT's Tim Wang and ...These lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9...Genetic Screening in Mammalian Cells with a Lentiviral CRISPR-Guide RNA Library. Koike-Yusa H, Li Y, Tan...

  2. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related ...using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses). Separating...related viral preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line... Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed..., can be expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression...requested lentivirus and AAV of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9... Cas9-expressing lentiviruses will no longer be carried over to the gRNA lentiviruses. This means that...

  3. Lentiviral Vector Uses and Overview

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    Blog Post
    ...the Addgene Blog Your Lentiviral Plasmid FAQs Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale... non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications, and they...or knockout cell lines. Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top ...Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our Lentivirus Guide Learn about... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods... systems, see our Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly...current (and future) applications of lentiviral vectors! Lentiviral vector history and components In the...

  4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ...purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing... is gradually reduced. For example, after using CRISPR/Cas9 to edit a gene, the expression of Cas9 is ...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology...check out Addgene's Lentiviral Guide). To produce lentiviral vectors, the lentiviral genome is distributed...how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production protocol...All Things Lentivirus More resources on addgene.org Lentiviral Guide   Popular lentiviral vectors ...integrase-deficient lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors...

  5. Your Lentiviral Plasmid FAQs Answered

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    Blog Post
    ...physiological difference between lenti- and retroviruses. Lentiviruses are able to enter an intact nuclear... Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr Library Resources...shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more. Not surprisingly, we ...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...frequently asked lentiviral questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier...Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral packaging...HIV-derived. Get Tips on Titering Your Lentivirus Concerning safety of lentiviral transduction Q8: You mentioned...

  6. Lentiviral Prep Service

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    ...Packaging Service page . Pooled CRISPR Libraries for Screening Use pooled CRISPR libraries to screen for genes...libraries 92385 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set A Human CRISPR inhibition library...Root 92386 Human CRISPR inhibition library Dolcetto in XPR_050 (VP64) Set B Human CRISPR inhibition library...genes. For more information on CRISPR plasmids and function, see our CRISPR plasmids resource page . Cas9...GFP Zhang 61425 lenti dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding...Pooled CRISPR Libraries Cas9 Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...

  7. CRISPR Library Amplification

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    Protocol
    ...amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.... Protocols CRISPR Library Amplification...Amplification CRISPR Library Amplification You may also like... Pooled libraries Guide to Using Pooled Libraries...Libraries All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library...following protocol can be used as a starting point for CRISPR libraries. This protocol allows the amplification...gel ( see restriction digest protocol here ). Lentiviral plasmids can recombine between their LTRs resulting...these sequences are not efficiently packaged into lentiviral particles. See below for options if the recombinant...

  8. Tips for Titering Your Lentiviral Preps

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    Blog Post
    ... Addgene Blog Read Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn...considerations for titering lentivirus Volume and transduction time will influence lentiviral titers as will the...prepped your DNA, transfected and harvested your lentivirus. Now it’s time to move ahead with your infection...defective viral particles. Direct measurement of lentiviral RNA is one alternative to direct p24 measurement...these universal primers can be used to titer any lentiviral prep that shares that specific feature. Primers...transfection; if preparing several different lentiviruses in parallel, this approach should be considered...transgene expression. The most accurate method of lentiviral titration uses qPCR to measure the number of ...

  9. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ... While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges...Talking 'bout my (lentiviral) generation The first step in delivering genes via lentivirus is deciding what...different generations of lentiviral packaging systems please see Addgene’s Lentiviral Guide. While the third...Producing lentivirus: it's all about the T's (transfection and titers) To produce lentivirus, the plasmids...producing lentivirus, it is time to titer. One important consideration when titering a lentivirus is the ...more tips on lentiviral titering please see our blog post. Between choosing the best lentiviral system, producing...

  10. Lentivirus Plasmids

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    ...technologies. Looking for lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids...plasmids table and search "lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...

  11. Getting the Most from Your Lentiviral Transduction

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    Blog Post
    ... How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org Find...Things Lentivirus with our Lentiviral Guide Pages Deposit Your Lentiviral Vectors ...for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus to be time...a successful lentiviral infection, happy virus is the second. When harvesting lentivirus, some labs prefer...success of your lentiviral transduction. The effect of freeze-thaw cycles on lentiviral stability depends... when the lentiviral stock needs to be titrated. If storing for less than a day, lentivirus can be kept... targets for a lentivirus are determined by the envelop protein. Many times lentiviral vectors are engineered...

  12. Quick Guide to All Things Lentivirus

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    Blog Post
    ...These properties make lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and...property makes the lentivirus an attractive choice for a gene transfer vector. Lentiviral vectors Although...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome ...used. Inducible lentiviral transfer vectors The most widely used inducible lentiviral vector system is...Your Research Lentiviral Vector Uses and Overview Resources on Addgene.org Find Lentiviral Vectors for...Ready-to-Use Lentivirus Browse Our Lentivirus Guide Pages  ...If you are interested in using lentiviral vectors to introduce your favourite gene into your favourite...

  13. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

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    Protocol
    ...bacterial strain Watch the Video! CRISPR Library Amplification Amplify CRISPR pooled-plasmid libraries Diagnostic...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

  14. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...for 1-3 days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead...vector and Lenti expression vector. Turn it on for just a little while Yes, there’s a CRISPR for that, ..., too! CRISPR activators (CRISPRa) are transcriptional activators fused to a dead Cas9. The system is ...approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR activator and repressor mechanisms... the Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...knock out the gene for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate... Promoters Plasmid 101: Repressible Promoters CRISPR 101: Which Cas9 Do I Choose for my Experiment ...

  15. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T cells...Pooled Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR... construction, lentiviral production, transduction, screening, and validation of CRISPR libraries. The...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Curr Protoc Mol Biol. 2016 Jul ...but we do distribute several of our most popular CRISPR pooled libraries and one of our most popular barcoding...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...The Genome Engineering using CRISPR/Cas Systems Forum- Moderated by the Feng Zhang lab, this is a useful...

  16. CRISPR Plasmids - Empty gRNA Vectors

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids...Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector Mammalian Lentiviral expression...S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...Yang pLCKO 73311 Mammalian/Lentiviral hU6 none S. pyogenes Puro Moffat lenti sgRNA(MS2)_puro backbone 73795...pyogenes URA3 Davis lenti sgRNA(MS2)_puro optimized backbone 73797 Mammalian/Lentiviral U6 none S. pyogenes...plasmids and therefore are not limited to a single CRISPR function. Alternatively, gRNA-only plasmids can...

  17. Hot Plasmids: Spring 2025

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    Blog Post
    ...Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene cloned EGFP into pAG Lenti CMV N-HA...#236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081), then generated lentiviral particles in HEK293T...editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the pithy name "site-specific...site-specific target-primed insertion through targeted CRISPR homing of retroelements." The Abudayyeh-Gootenberg...tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans...very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's...Addgene #12260). HeLa cells were infected with 96 h lentiviral supernatant and selected with puromycin. Puromycin-resistant...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ... Mammalian lenti sgRNA(MS2)_zeo backbone - 3rd gen lenti sgRNA cloning backbone with MS2 loops at tetraloop... Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian...for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection, and browse our... genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO ...pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by...

  19. Virus Protocol - Generating Stable Cell Lines

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    Protocol
    ... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature...cells that have taken up the lentiviral transgene. Note that not all lentiviral vectors deliver antibiotic...Thaw the lentiviral aliquot on ice prior to use. Prepare a range of dilutions of the lentivirus in DMEM.... This is because lentiviral integration is random. Given the MOI of the lentivirus used, cells may also...an integrated lentiviral vector. Protocols... Protocols Generating Stable Cell Lines with Lentivirus Generating Stable...Stable Cell Lines with Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols...

  20. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post
    ...outperformed another CRISPR library with 6 sgRNAS, highlighting the benefit of sgRNA design in CRISPR libraries.... about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled CRISPR libraries...genome-wide screening using CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here ...genome-wide CRISPRko, CRISPRi, and CRISPRa libraries have been shown to outperform other popular CRISPR libraries...repressor (CRISPRi) or an activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries...generation libraries for genome-wide CRISPRa, CRISPRi, and CRISPR-Cas9 knockout genetic screens." bioRxiv...advantages of the optimized human CRISPR libraries. Brunello: human CRISPRko sgRNA library The Brunello library...
Showing: 1 - 20 of 123 results