...requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G...of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (...founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono lab ...envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini...top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid... producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as pCMV-dR8.2...pMDLg/pRRE - This is a 3rd generation lentiviral packaging plasmid from the Trono lab that contains the...

...pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particles...for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such ...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster,...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...

...TRC cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those...those are lentiviral constructs. In 2007 we continued to see requests grow for lentiviral packaging and expression...expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier Trono's lab are...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...our stride. We shipped our 100,000th plasmid. A variety of plasmids continued to be popular years after...pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome...

...the transfer plasmid, a plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral... cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell culture...regions of the transfer plasmid.   Lentivirus resources Browse lentiviral preps available from Addgene...Concentrated lentiviral preparations (“-LVC” catalog items) are generated from lentiviral preparations...being frozen. Specifically, lentiviral particles are collected from the lentiviral preparation by precipitation...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR.... coli. In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits.   ...

...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer ...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...-VSV-G N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains...Addgene lentiviral packaging, envelope, and transfer plasmids Viral... Viral Plasmids Lentiviral Plasmids Lentiviral...

...-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...Envelope and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...

...Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your insert...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3... Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect packaging cells Day 2 (am): 18 h post-transfection...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ... cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be...

...the transfer plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral... cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell culture...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... coli . In addition, plasmids are typically prepared using endotoxin-free plasmid purification kits. Resources...our virus protocols . Browse our plasmid collection of popular lentiviral vectors ....AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...

...μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-... shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing ...to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio that gives....1 can also be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles...Due to the long terminal repeats found in lentiviral plasmids, Addgene currently recommends using a strain...puromycin concentration F.3 Protocol for lentiviral infection and selection G. Safety H. References H.1 Published...