... all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono ...Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid from the Didier Trono lab that expresses...It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such ... lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver... for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as...

...pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope expressing...producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as ...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particles...Akitsu Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information...applicable export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks...

...those are lentiviral constructs. In 2007 we continued to see requests grow for lentiviral packaging and expression...expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier Trono's lab ...such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs ...

...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell culture...protocols. Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ("-LV” catalog... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...

...12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...plasmid ). This table lists common lentiviral envelope and packaging plasmids that can be used with 2nd...additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase deficient lentivirus...

...-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...γ-Retrovirus Envelope and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid...12371 pCL-Eco Packaging Contains MoMLV gag, pol, and env Verma 35614 pBS-CMV-gagpol Packaging MoMLV gag and...Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope VSV-G-expressing...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...retroviruses called γ-retroviruses. γ-Retroviruses can package relatively high amounts of DNA (up to ∼8 kb), and...

... #236081), then generated lentiviral particles in HEK293T cells with pMD2.G (Addgene #12259) and psPAX2...tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans...fusions for membrane display, they recommend the NGR vectors for most experiments. These new tools promise to... toolkit is the empty plasmid backbone: the DNA vector that contains everything needed to replicate inside...very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's...taken them for a test drive! We validated viral packaging and strong expression after inserting EGFP and...launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! Figure 2: Addgene...

...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector is 8901 base...pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free...Producing Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles...-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The ...TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing...scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral...

...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR... cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell culture... . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced... Viral Vector Packaging Service Viral Production Viral... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...

...Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer...from a lentiviral vector transfected into 293T cells using a polyethylenimine (PEI) transfection protocol... Timeline Day 0: Seed 293T packaging cells Day 1 (pm): Transfect packaging cells Day 2 (am): 18 h post-transfection...polyethersulfone (PES) filter Transfection grade DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer...Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus from...for alternative packaging cell lines or transfection reagents. Once produced, lentivirus can be used for...Considerations Before You Start The health of the packaging cell line is critical for obtaining high viral...