...pENTR-Cre Cre with stop codon Entry vector Ko 139546 pENTR-CreStopDeleted Cre without stop codon Entry vector...Mammalian Green 8406 p252 pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green...Green 8408 p255 pPr-CREM/Ins/CMV-STOP-luc CREM and CMV-STOP-luc cassette, separated by insulator probasin...Expression: Placing a stop codon with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette...Lentiviral Jacks 11584 Lox-Stop-Lox TOPO Cre activates gene of interest by removing Stop sequences; Puro selection...pCMVe-betaAc-STOP-luc Cre dependent luciferase expression Mammalian Green 32145 pJFRC172-10XUAS-loxP>-dSTOP-loxP...vector Ko 139547 pENTR-CreERT2 Cre-ERT2 with stop codon Entry vector Ko 139670 pTFUbiCre Cre Maize Ubiquitin...

...presence of Cre, the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is...with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest...is from Tyler Jack's lab: Lox-Stop-Lox TOPO.  Cre-dependent gene knockout – conversely, putting the loxP...canonical sequence. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant...

...recombinase-dependent vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF) sequence prior to an ATG start...loxp site and one lox2272 site on either end of the transgene. Since loxp and lox2272 can not recombine...are comprised of different sequences (loxp and lox2272) and can not recombine with each other. When Cre...orientation for transcription. Upon recombination one of lox2272 sites will now be sandwiched between 2 loxp sites...binds to either pair of lox sites, let's say the lox2272, that are in the opposite orientation (direction...the loxp site is excised since it is between two lox2272 sites in the same orientation preventing the transgene...