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We narrowed to 40 results for: luciferase reporter

Showing: 1 - 20 of 40 results

  1. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...expressing luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ... engineered luciferases Although firefly luciferase was the first bioluminescent reporter to be cloned... scientific community: luciferases, which have become common genetic reporters and alternatives to fluorescent...catalyzed by luciferase, releasing light. Created with BioRender.com. Bioluminescent reporters require ...improved signal sensitivity. Another advantage: luciferase reporters aren’t susceptible to photobleaching — they...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...

  2. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post
    ...researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase to excite the ...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs were designed to be packaged...furimazine substrate for NanoLuc luciferase. Both GpNLuc and OgNLuc BRET reporters produced bright signals without...excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking two CyOFP1...

  3. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require... imaging tools The luciferase reporter gene The green fluorescent protein reporter gene Resources on ...developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time... time, but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome these limitations...existing bacterial reporters Limited Depth of Signal/Imaging: Fluorescent reporters are not as well suited...fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint what...signal is coming from.   Acoustic reporter genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene...

  4. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have...fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...their reporter mRNAs. Using the TRICK plasmids, Halstead et al demonstrated that 91% of reporter mRNAs...expressing a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final...customized fluorescent reporters can be challenging enough, but incorporating multiple reporters into a single .... Nat Methods. 2011. High-throughput luciferase reporter system for detection of pre-mRNA splicing...

  5. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... of its target gene, in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts...the promoter of the reporter gene to activate expression (Figure 2c). The reporter gene then allows detection...genes as reporters and some that repress instead of activate transcription. The HIS3 reporter system (... that avoid transcriptional reporters altogether; In the split-luciferase system, intein-mediated splicing...reconstitutes functional luciferase only when fusions of the two halves of the luciferase protein interact and...co-expressed in a yeast strain that also contains a reporter gene cloned downstream of a promoter containing...referred to as the “bait”, Figure 2b) cannot activate reporter gene expression on its own, but, if the two POI...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...gene disruption in M. abscessus  Bioluminescent reporter of mechanical forces   Epitope tag antibody ...Western Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke...doi.org/10.1128/jb.00335-23.  Bioluminescent reporting of mechanical forces with PILATeS By Emily P. ...mechanosensor called PILATeS, Pulling-Induced Luciferase Association Tension Sensor (Zhong et al., 2024...sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded in the I10 domain of the human titin...tension. When this happens, BiT and the LgBiT luciferase fragment (expressed separately) assemble and ... reaction (Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal...

  7. Luminescent Imaging with Nano-lanterns

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    Blog Post
    ...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers...problems of low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently...Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology...transfer (BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent...need for an excitation light source. Instead, a luciferase substrate like coelenterazine is supplied in ...eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...

  8. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...replaced by a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific...as a template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase recognizes the...viral infection), producing firefly luciferase. Firefly luciferase can readily be measured to quantify...necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase (reviewed...start signal and initiates transcription of the reporter gene. The polymerase will elongate the mRNA until...

  9. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance... editing. eGFP reporters for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9...DSB in their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target...editing reporters. Image from St. Martin et al., 2019. The Harris lab used these eGFP reporters to analyze...Transient reporter for editing enrichment (TREE) The Wang and Brafman lab developed a transient reporter for...cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid delivery... functional reporter system to identify and enrich for base-editing activity The reporters mentioned above...

  10. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ... transformation. Choose good reporter genes There are many great reporter genes available and which one...confirm transformation success. Luciferases are great quantitative reporters and use a substrate to produce...the T-DNA region with our gene of interest or a reporter gene so that the transformation and gene expression...Once we were on the right track, we switched to luciferases to more easily quantify the effect of the different...different treatments on transformation success. Some reporter gene examples are below. The GUS gene (β-glucuronidase...histochemical GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate...produce bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore...

  11. Luciferase Plasmid Collection

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    Collection
    ...CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...respectively), Cypridina luciferase , and Luciola luciferase . Each type of luciferase has advantages and disadvantages...Multiple Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b,...collection of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid...Collections Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent ...

  12. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry into...cells! After verifying the specificity of their luciferase assay, they concluded that components in the ...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

  13. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post
    ... and a UAS-driven luciferase reporter construct. In the absence of GFP, no reporter output was observed... and a reporter construct into the mouse retina. GFP expression robustly activated the reporter gene TdTomato...and Cre-DOG, respectively, repurpose popular GFP reporter lines for more sophisticated experimental manipulations...create a complete transcription factor, activating luciferase transcription. Tang et al. coined this system...GFP. They then tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells ...tested in retinal electroporation studies, DsRed reporter expression was induced in ~76% of GFP+ cells, ...

  14. Promega Plasmid Collection

    Type
    Collection
    ...HiBiT, and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...experimental workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...search by response element, reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "NanoLuc", "HaloTag...NanoLuc luciferase-fusion protein expressed in cells. Additional Resources Browse Addgene's Luciferase Plasmid...Addgene Find additional information about luciferase reporter assays (Link opens in a new window) from ... 19.1 kDa and exceptionally bright and stable luciferase that enables highly sensitive detection at endogenous...

  15. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing...expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing...EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase with neomycin...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing...expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral...

  16. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...light include chemiluminescent proteins, like luciferases, as well as fluorescent ones, like Green Fluorescent...responsible for encoding GFP, and, in 1992, he reported its sequence. Soon after that, in 1994, Prasher...he lived! His hunch paid off, and he was able to report new fluorophores like DsRed from Discosoma sp. ...

  17. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...TALEN array for genomic engineering Luciferase Reporter See our Luciferase collection of plasmid backbones... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ... genes can be inserted into this firefly luciferase reporter to test for their effects on protein production...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more...page for more information Genome Modifications, Reporter Assays, mRNA Regulation, and More Find empty backbones...backbones for other uses such as genome modification, reporter assays, shRNA expression, transgenics, and more...

  18. Bacterial Expression Systems

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    Collection
    ...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...

  20. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...protein function, thereby creating a single-molecule reporter of intracellular processes. Initially, Tanenbaum...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...
Showing: 1 - 20 of 40 results