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We narrowed to 56 results for: mcherry reporter

Showing: 1 - 20 of 56 results

  1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance... 2018). The ACE reporter is a bicistronic construct that consists of a mutated mCherry and a downstream...: The ACE reporter from the Harris Lab relies on the restoration of a frame-shift in mCherry that restores... editing. eGFP reporters for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9...DSB in their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target...downstream of wild-type mCherry and a T2A site and expressed in cells. mCherry is constitutively expressed...dividing the number of eGFP and mCherry positive cells by the number of only mCherry positive cells (Martin et...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...cells.  Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems provide... CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying...histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct... 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does GEmCherry2 work? The...focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover single base-pair...iteration was named GEmCherry1, due to the “genomic fragment inserted into mCherry”. However this one ...AAVS1 locus using GEmCherry2. In this experiment they saw a clear difference in mCherry expression using...

  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon...pAAV-hSyn-DIO-mCherry Bryan Roth 50457 AAV1 pAAV-hSyn-DIO-EGFP Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl...Flp-dependent expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898...pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry Connie Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell... Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...Serotype Name 44362 AAV1 pAAV-hSyn-DIO-hM4D(Gi)-mCherry 83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell...

  4. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...these experiments.  AsLOV2 Inertion Site mCherry #1 mCherry #2 GFP Loop 1 dark-induced dark-induced...Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies affected binding of their target... its co-localization with a membrane-localized mCherry or GFP protein. With this system, if the OptoNB...sites in all eight surface-exposed loops of an mCherry nanobody. Only insertion into loops 1 and 6 resulted...scheme to generate two more OptoNBs, one against mCherry and one against GFP. Insertion of AsLOV2 into loop...which revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead, suggesting... a membrane localized GTPase, the team fused a mCherry OptoNB to the catalytic domain of the Sons of Sevenless...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from ...Spatio-temporal expression of optogenetic reporters: Since most optogenetic reporters are delivered using AAV injection...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ...Cre-dependent reporter expression, the potential for background expression of the reporter will be greatly...that turns BFP expression off, while turning on mCherry expression. For this FLEx switch to successfully...sequence in the sense orientation, followed by the mCherry coding sequence in the antisense orientation (Figure... switch will express BFP in the absence of, or mCherry in the presence of Cre recombinase.   Once ...

  6. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...and the DNA-binding domain of AraC using a mCherry reporter downstream of a PBAD promoter. While none ..., contains BLADE, the PBAD promoter, and a mCherry reporter downstream of the PBAD promoter. To use this...created two constructs, among others: pBLADE-mCherry: This construct, built from the pBAD33 backbone... with your gene of interest, you would replace mCherry with your gene of interest. pBLADEONLY: This plasmid...with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They created a bacterial...

  7. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry fluorescence...edits out a frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies simply...and N177F mutations). Its excitation maximum is reported as 603 nm, while the emission maximum is 675 nm... ACE system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9...editing tools, the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based...simply involves tracking the ratio of mCherry-positive to eGFP positive cells through fluorescence microscopy...containing rat APOBEC1. Finally, they show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting...

  8. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral ...114472  AAV5, AAV8  pAAV-hSyn-mCherry 50459  AAVrg  pAAV-hSyn-DIO-mCherry 59462  AAV2  pAAV-CAG-tdTomato...with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal electrical activity...Serotype Name 75033  AAV1  pAAV CD68-hM4D(Gi)-mCherry (CD68 is a  microglia/macrophage promoter) 121539...pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Calcium...

  9. New Viral Vectors - March 2024

    Type
    Blog Post
    ... pAAV-CaMKIIa-mCherry  AAV PHP.eB Controls Deisseroth New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls...New viral vector pAAV-nEF-Con/Foff DREADD Gi-mCherry AAV8 Chemogenetics Deisseroth New viral vector ...check out Addgene's Data Hub to view viral vector reports from other users. And don't forgot to use the link...

  10. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...TRICK system, the 3’ UTR of the reporter mRNA is labeled by PCP-mCherry (Figure 2). The 3’ UTR also contains...proteins to allow translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the...GFP protein that binds to the coding region of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) an RFP...translation, both GFP and RFP are bound to the reporter, causing it to appear yellow. The act of translation...of the mRNA, leaving only a red FP bound to the reporter. Because it contains a nuclear localization signal...copies of the FP-coat protein fusions to their reporter mRNAs. Using translation inhibitors cycloheximide...

  11. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...both mCherry and TagRFP.  Here, the TagRFP spectrum is shown in the darker colors and the mCherry spectrum...larger fraction of the TagRFP emission than the mCherry emission. For this filter set, we would expect ...expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 ...or mRuby2 than longer wavelength proteins like mCherry. For background on fluorescence and filter sets...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

  12. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...fluorescent CatCH-GFP reporter. Expression of GFP will only result in cells where Cre-mCherry is also expressed...expression of the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP ... using one promoter to drive expression of Cre-mCherry and a second promoter to drive expression of a ...expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP expresses...cells transduced by both AAVs would express Cre-mCherry and GFP.   Figure 2: Synthetic promoters ...

  13. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-CaMKIIa-hM4D(Gi)-mCherry AAV1 Chemogenetics...pAAV-hSyn-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics...help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep...Control Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...Control Gordon Fishell New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...Control Gordon Fishell New viral prep pAAV_BiVIPe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell ...Control Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell...

  14. Hot Plasmids - February 2022

    Type
    Blog Post
    ...4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG...while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG frankenbody...induce high levels of gene activation for both reporter and endogenous genes. It is highly specific in...

  15. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...gene disruption in M. abscessus  Bioluminescent reporter of mechanical forces   Epitope tag antibody ...Western Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke...traditional homologous recombination methods). By using mCherry for selection of sgRNA plasmid transformants, they...with the sgRNA cassette on a plasmid expressing mCherry and resistance selection marker HygR. The CRISPR...disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying ...doi.org/10.1128/jb.00335-23.  Bioluminescent reporting of mechanical forces with PILATeS By Emily P. ... reaction (Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...in the absence of Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes...recipient’s initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... the 3’ UTR of the reporter mRNA via an MS2 coat protein (NLS-MS2-RFP) the reporter mRNA, which contains...their reporter mRNAs. Using the TRICK plasmids, Halstead et al demonstrated that 91% of reporter mRNAs...customized fluorescent reporters can be challenging enough, but incorporating multiple reporters into a single ...pyralis) reporter plasmids for use in high-throughput screening of pre-mRNA splicing. This reporter system... ER mCherry-Sec61 beta BFP-Sec61 beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin...mito-BFP mCherry-Drp1 (GTPase in division) GFP-Mff (outer membrane protein) Late Endosome mCherry-Rab7A...widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been...

  18. Hot Plasmids - August 2020

    Type
    Blog Post
    ...and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells are then cultured...the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9...promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system, they could quantify and track...dCas9 targeting plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500...mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. Find these AAVs at Addgene ...

  19. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...designed by putting together the coding sequence of a mCherry fluorescent protein followed by a stop codon and... flow cytometry is used to sort populations by mCherry and GFP expression to check for ABE’s. b) Quantification...

  20. Hot Plasmids - January 2023

    Type
    Blog Post
    ...promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the weaponized...
Showing: 1 - 20 of 56 results