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  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...the teams at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary mission ...with ComSciCon, find us on Twitter, Facebook, Instragram, or send an email to info@comscicon.org. Many...guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference series for...are passionate about communicating science.  ComSciCon's events have 3 main goals: To help graduate students...communication from across the US and Canada convene at ComSciCon's annual flagship workshop.  Our 2018 workshop ...activities of our attendees there online with #comscicon18 and stay tuned to our website and social channels...regional and disciplinary communities gather at ComSciCon's local and specialized franchise workshops throughout...
  2. Finding Your Perfect Job After University

    Type
    Blog Post
    ...I decided to apply to study a Master of Science (MSc) in Human Molecular Genetics to give me an edge over...employers were requesting that only scientists with an MSc or higher apply. It was important to weigh the cost...you could not receive government loans to study a MSc, so I had to take out a personal bank loan. I saved...and took evening work to help cover the costs. My MSc courses were interesting and luckily genetics is ... a genetics related role soon after finishing my MSc. The masters program was 6 months of classes and .... Working in industry: virology On completing my MSc, I again had to look at what type of work I would...
  3. Important Considerations When Using AAVs

    Type
    Blog Post
    ...guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University of Manitoba. Location,...Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at the University of Manitoba. Her project...
  4. Lentivirus Plasmids

    Type
    Collection
    ... GP and MLV 4070A Env GP. Good for work in human MSCs. Use packaging construct encoding Tat. Reiser Return...
  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and mScarlet-I3 constructs. Scale bar...hours. In order to use the latest mScarlet tags, mScarlet3 and mScarlet-I3, Oliver Hobert’s lab recently...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ...like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet’s slow... The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-optimized for C. elegans...structural studies of E3 ligase cereblon Optimized mScarlet-based tags for live imaging in C. elegans CRISPR...s41467-024-52871-9. PMID 39406745. Optimizing mScarlet tags for live imaging in C. elegans By Alyssa ...
  6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...protein (mScarlet) with either a seven or eight amino acid linker between them. The Ace-mScarlet voltage...results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive...inhibitory rhodopsin Ace2N and mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image ...created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin...blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et...
  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I with T74I (...compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H with M164H...compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet variants...New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed ...synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a combination of deliberate...protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat...Methods. 2017. PubMed PMID: 27869816 Listen to our mScarlet Podcast Segment    Photocleavable protein PhoCl...
  8. Hot Plasmids - October 2020

    Type
    Blog Post
    ...https://doi.org/10.1016/j.isci.2020.101330 Split-wrmScarlet, a fluorescent proteins system for Caenorhabditis... elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is three times brighter...Calico Life Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments that are not ... engineered C. elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. ...with a ssODN template to introduce the small wrmScarlet11 which is less than 60 nt in length to tag the...distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different subcellular ...
  9. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV5 Optogenetics...Ofer Yizhar New viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE AAV1, AAV5 Optogenetics Ofer Yizhar New...Edward Boyden New serotype pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE AAV1 Optogenetics Karl Deisseroth New serotype...
  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...Expression Type MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II...IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral...
  11. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N...Ace2N quenches a proportion of mScarlet's emission via FRET.  Image from Beck et al, 2019 under CC BY 4.0...
  12. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...
  13. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ...
  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...derived from MoMLV (Moloney Murine Leukemia Virus) or MSCV (Murine Stem Cell Virus) sequences. Packaging genes...viruses were derived from different genomes (MoMLV and MSCV for γ-retrovirus; HIV for lentivirus). Additionally...
Showing: 1 - 20 of 55 results