...tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli...SECURE base editors All-in-one AAV delivery of Nme2Cas9 with gRNA cassette Erik Sontheimer’s lab identified...identified a Cas9 protein from Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC...high target site density (average every 8 bp). Nme2Cas9 is also small enough that it can be packaged into... into AAV. The lab used single-AAV delivery of Nme2Cas9 and the sgRNA to demonstrate efficient editing.... Read the paper in Molecular Cell Find the Nme2Cas9 All-in-One AAV constructs CRISPR interference...