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We narrowed to 477 results for: organization Addgene

Showing: 1 - 20 of 477 results

  1. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...designed by the Addgene nonprofit plasmid repository and is being shared outside of Addgene for informational...is for labs that work with pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae...administrators, or anyone else associated with Addgene, can be held responsible for your use of the information...

  3. CRISPR Library Amplification

    Type
    Protocol
    ...Molecular Biology Reference How do I process my Addgene pooled library? Introduction Please refer to our...depositor for a specific pooled library available at Addgene. We recommend following the depositor's specific...DNA: guide RNAs targeting different genes in an organism's genome, for example. Amplification is usually...results. If you obtained the pooled library from Addgene, these protocols can be found on the Pooled Library...with ice and water to create slurry) 100 mL LB At Addgene we use premixed LB (VWR 101414-072) for convenience...Help Center to get details on how to process your Addgene pooled library...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...Addgene's zebrafish resources: Find plasmids for use in zebrafish....been established as a powerful vertebrate model organism for the study of genetics and developmental biology...highlight some of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools...containing your zebrafish gene of interest? Search Addgene's collection for plasmids that contain a zebrafish...Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR...Multicolor fluorescent labeling in zebrafish Whole organism lineage tracing by combinatorial and cumulative...screening fluorescent protein fusion expression in organisms or cells in which an exogenous mRNA can be injected...

  5. Microbiology Resources

    Type
    Collection
    ...yeast plasmids? Addgene has a wide variety of tools for work in these model organisms. See below for plasmid... post to see how Addgene depositors are making this possible in a range of organisms. Find CRISPR tools...Protein Guide : Addgene's extensive fluorescent protein plasmid collection is organized by application ...Addgene's microbiology resources: Find plasmids for your microbe of interest, including bacteria, viruses... Plasmids Microbiology Plasmids and Resources Addgene’s repository contains plasmid tools for a range ...additional microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and distributions...

  6. CRISPR Plasmids and Resources

    Type
    Collection
    ...below by function. Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...zebrafish, C. elegans, parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background... on our popular CRISPR 101 blog series, we've organized a comprehensive CRISPR resource for you to download...target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR plasmids designed for use in bacteria...

  7. Distribution to Industry

    Type
    Collection
    ...affiliate it with your organization. If your organization has not ordered from Addgene in the past, we will...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...by phone, fax, or email. Pricing Addgene is a nonprofit organization dedicated to providing the scientific...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...A portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the ...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...

  8. Cancer Research Plasmids and Resources

    Type
    Collection
    ...Addgene's curated collection of plasmids for cancer research, organized by signaling pathway. ...Research Cancer Research Plasmids and Resources Addgene has a large variety of resources for use in cancer...constructs. Each collection page listed below organizes the plasmids and resources by signaling pathway...

  9. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...upregulating gene expression in various model organisms.... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  10. Fluorescent Proteins: FRET

    Type
    Collection
    ...Fixed Cells Additional Resources at Addgene Learn more on the Addgene Blog: Introduction to FRET and Tips... on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules...can be influenced by many factors, such as the organism or cell type, relative position and orientation...Experiments . Find many FRET-based biosensors in Addgene's Biosensors Collection . Generate custom FRET-based...FRET pairs and biosensors can also incorporate organic fluorophores via HaloTag or SNAP-Tag . Read more...Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection or the Promega Plasmid...

  11. Worm Expression Resources

    Type
    Collection
    ...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR... “the worm”) is widely used as a genetic model organism due to their rapid life cycle (3 days from egg...highlight some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools...of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description Gene...Biology Synthetic biology constructs - Browse Addgene's collection of synthetic biology plasmids for use... in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR...methods to engineer the genome of the genetic model organism C. elegans. Return to top Do you have suggestions...

  12. CRISPR References and Information

    Type
    Collection
    ... Information Addgene Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources...Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR...screening. CRISPR eBook Addgene's downloadable eBook about all things CRISPR. Addgene CRISPR blogs CRISPR ... and news published on the Addgene blog. CRISPR Pooled Libraries Addgene's collection of CRISPR pooled...protocol Addgene CRISPR pooled library amplification CRISPR pooled libraries Protocol at Addgene Church ...Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR...activation, inhibition, and barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software...

  13. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...E-coli-as-a-Model-Organism.aspx. Additional resources on the Addgene blog: Read more about model organisms in the...covered five model organisms in a previous blog post, but here are five more model organisms that have left...emerging model organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia...as a model organism for over 60 years with a status as the most studied prokaryotic organism. With its ...learned more about popular model organisms in the lab, check out Addgene’s plasmid collections to help start...Undervalued Model Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–...and molecular tools. One such tool is the model organism, an essential aspect of biological research that...

  14. Five Popular Model Organisms

    Type
    Blog Post
    ...Lukas Morgan Model organisms are vital tools used by researchers around the globe. These organisms share... find these plasmids at Addgene. Another advantage of mice as model organisms is that they are genetically...one of the simplest eukaryotic organism, is a commonly used model organism in scientific research. Yes, ...resources check out Addgene’s Worm Expression page. Looking for more model organisms? Check out Part two...model organisms series, and read about new systems being developed for emerging model organisms! References...this blog post, we’ll cover five popular model organisms, but there are many more out there. The house... start with the most preferred mammalian model organism of many researchers: the mouse (Mus musculus)....

  15. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate and thoughtful...opportunities within an organization but outside your comfort zone As part of my position at Addgene I am now involved... outside an organization The next step is to expand your efforts beyond your organization and/or scientific...important? In my current job at Addgene I work with people from various organizations in science and beyond. For... Science Training Resources on Addgene.org Check Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly...

  16. The MTA at Addgene

    Type
    Blog Post
    ...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority ...Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over the last twenty...differs.  When you deposit a plasmid with Addgene, your organization’s tech transfer office is sent a deposit.... In signing this agreement, your organization agrees to let Addgene receive, store, and distribute your...recipient organization is sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move...distribution for their plasmids. MTAs at Addgene Since Addgene was founded in 2004, we’ve handled the majority...If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer...

  17. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...genetic code is used by most organisms on Earth, but different organisms have different preferences for...codons are used preferentially in which organisms - organisms have codon usage bias. You can find many...ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene that’s been codon ...peptide of interest and produce that peptide in your organism of choice. Unfortunately, codon preferences make...and expect your sequence to express well in any organism. So what are the evolutionary constraints that... do about them? Read on to find out!   Why do organisms have different codon usage biases? The reasons...reasons for varied codon preferences among organisms aren’t completely understood, but some possible reasons...

  18. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...Resources on Addgene.org Check out Addgene's synthetic biology page Search for a plasmid Find Addgene's most ...coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene as a large...was moved exactly as is from one organism to a distantly related organism, the gene may not express well...distantly related organisms. In 2006, less than 1% of the genes deposited at Addgene were synthetic. But...or intentional release of engineered organisms. “Is [the organism] engineered? Where did it come from?...large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes...this lies in the codon usage biases of different organisms. Different species have different preferences ...

  19. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...on the Addgene blog Check out our other model organism blog posts: Five Popular Model Organisms, Part...this programs via Addgene. New organisms for genotype to phenotype studies New organismal systems described...diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...and share all of these new organisms continues to be challenging. Organism specific viral vector systems...and shared for many different organisms. Do you have a favorite organism you study in the lab? Let us ... work but never get to actually manipulate the organism they study (unless if you count normal human interaction...spiny purple sea urchin. It’s not a standard model organism, but it was easy to harvest eggs and sperm, mix...

  20. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...Industry Additional Addgene Resources Blog: Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids... viral proteins in several expression systems. Addgene partnered with Ginkgo to make these plasmids available...available to scientists in academic and industrial organizations. For more information on ordering from a for-profit...expressed or functionally validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to...
Showing: 1 - 20 of 477 results