...first-generation mARGs also had a large genetic footprint (two cassettes + a booster cassette), their expression...mARGs with higher expression, a smaller genetic footprint, and nonlinear acoustic scattering. The best candidate... Increasing expression and decreasing genetic footprint We took a cue from the native expression patterns...cassette, either, allowing us to reduce the genetic footprint yet again. This also led to improved expression...

...transposon gene modification system and the scarless, Footprint-Free™ removal of cargo with the excision-only ...thereby leaving behind only those cells with a Footprint-Free™ genome edit.   Figure 2: Editing...nuclease to stimulate homologous recombination and Footprint-Free™ removal of the selection cassette by excision-only...

...excises the transposon from the donor DNA. A DNA footprint is left behind. When the transposon-transposase...dinucleotides and it leaves behind the CAG DNA footprint from its terminal sequences at the excision site...other transposons, it does not leave behind a DNA footprint sequence after excision. piggyBac can mobilize...

.... Viral vectors also have the largest genomic footprint of iPSC generating methods due to the risk of ...Non-integrating methods have a smaller genetic footprint compared to integrating approaches. These methods...

...homozygous mutants with no co-integrated markers or footprints, and can be scaled up for systematic editing ...

...Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467...

...editing efficiency and reduced RNA-off target footprint compared to current N-terminal linked SaCas9 ABE...

...kits, but you’d like to reduce your lab’s waste footprint. The Zhao and Deng labs developed a simple method...