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Showing: 1 - 14 of 14 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    .... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab...to deliver Cas9 and your gRNA of interest into mammalian cells. This vector was the top requested plasmid...) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9 and your gRNAs.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...scrambled shRNA is used as a negative control for mammalian RNAi experiments.  Find the scrambled shRNA vector...2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your...

  2. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been...been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a blue flame for its adenine...Targeting: This page contains Cas13 constructs for mammalian and bacterial systems. Plasmids with wild-type...unique non-specific RNA cleavage in bacteria. In mammalian systems, Cas13a degrades only the specified RNA...anyone predict that we’d be able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now...Genotyping Day 5: Delivery systems: CRISPR 101: Mammalian Expression Systems and Delivery Methods and A ...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For...Web resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium (TRC...resistance gene for selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR 3’ Self-inactivating long terminal...lab. For more information, visit Addgene’s Mammalian RNAi Tools page. Several other laboratories have ...For more information, visit Addgene’s Mammalian RNAi Tools page. E.2 Protocol for Producing Lentiviral ...

  4. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including pcDNA-dCas9-p300...Tet1-CD for targeted cytosine demethylation in mammalian cells. This plasmid is used with pcDNA3.1-MS2-...DNMT3A-PuroR for targeted cytosine methylation in mammalian cells. Co-expression markers EGFP and PuroR enable.... Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9-D3A) constructs...vector in chick embryos, it also functions in mammalian expression systems.   Christopher Newgard’s Lab...Jaenisch R (2016) Editing DNA Methylation in the Mammalian Genome. Cell 167:233–247.e17 . https://doi.org...

  5. Cre-lox system

    Type
    Collection
    ...PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401 p224...Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII) CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused...cTnT Mammalian Campbell 89573 pCAG-iCre iCre CAG Mammalian Wong 89575 pCAG-VCre VCre CAG Mammalian Wong...Simpson 106368 pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal...split-Cre CMV Mammalian Hirrlinger 106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger... CAG Mammalian Scholer 125574 pCAG-Cre Cre CAG Mammalian Imai 125577 pCAG-iCre iCre CAG Mammalian Imai...expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch plasmid Mammalian Green 22799...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells ...pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase Mammalian Cells pDULE-ABK pyrrolysyl-tRNA synthase for...for aliphatic diazirine amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase for p-azido-l-phenylalanine...

  7. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...Description 47948 3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis... elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48880 3xFNLDD/pMXs-I2...3xFNLDD in mammalian cells 48972 3xFNLDD/pMXs-IG iChIP plasmid, expresses 3xFNLDD in mammalian cells 49536...3xFNLDD in mammalian cells 51240 3xFLAG-dCas9/pMXs-puro Expresses 3xFLAG-dCas9 in mammalian cells for ...3xFLAG-dCas9/pMXs-IG Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-I2 Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-neo Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...

  8. Genetic Code Expansion

    Type
    Collection
    ...164080 pCMV-MtPylRS (human-opti) PylRS M. thermophila acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS... mazei Mammalian TAG Edward Lemke 223509 pCMV_NESPylRS(WT)_hU6tRNAPyl PylRS M. mazei Mammalian TAG Edward...(Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang...71403 pCMV-DnpK PylRS M. barkeri N6‐(2‐(2,4‐dinitrophenyl)acetyl)lysine (DnpK) Bacterial, Mammalian TAG ...91706 pCMV-MbPylRS(DiZPK) PylRS M. barkeri photocrosslinkers DiZPK, DiZSeK, or DiZHSeC Mammalian TAG P...AbK Mammalian TAG Simon Elsaesser 140011 pAS_4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG ...-aminotyrosine Mammalian Huiwang Ai 154762 pAS_FLAG-Mma PylRS PylRS M. mazei Mammalian TAG Simon Elsaesser...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity; mammalian expression vector...constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1 expression plasmids, FRET constructs...method to increase CRISPR editing efficiency in mammalian cells. Their marker-free coselection, analogous...inhibit Spy Cas9 function in both bacteria and mammalian cells. These CRISPR inhibitors may provide a layer...reduce off-target gene editing. The bacterial and mammalian expression vectors for these CRISPR inhibitors...Expression Construct SpCas9 Inhibition (E. coli) Mammalian Expression Construct SpCas9 Inhibition (Human ...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C...158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C...GFP Mitochondria ABCb10 GFP Orian Shirihai 158003 pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-...Mitochondria-Outer Membrane Mff acGFP1 Gia Voeltz 158007 pCMV-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois...pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez...anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N-10 Golgi apparatus MannII mGold Francois... Late endosomes RAB7 DsRed Richard Pagano 158006 pCMV-mGold-Lysosome-N-20 Lysosome Lamp1 mGold Francois...

  11. Lentivirus Plasmids

    Type
    Collection
    ...plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd...and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev,...gene encoded by the transfer plasmid is toxic to mammalian cells, it can be difficult to efficiently produce...

  12. Retrovirus Plasmids

    Type
    Collection
    ...pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454 pCMV-VSV-G Envelope Envelope protein; use with packaging...inducible expression of GFP T2A Cre fusion in mammalian cells Foijer 47916 pRXTN CMV/MSV pRXTN is a modified...-Luc MoMSV Stable expression of luciferase in mammalian cells Wong Return to Top Do you have suggestions...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ... CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...that has been codon optimized for expression in mammalian systems. Other luciferases in our collection include...has also been codon optimized ( CBR2opt ) for mammalian expression systems. Each type of luciferase has...noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description...target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase... expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 64784 pGL3-Basic-IRES Firefly...Eric Campeau 18964 Luciferase-pcDNA3 Firefly CMV Mammalian expression of firefly luciferase William Kaelin...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Ding 186012 pCMV Flag-LRRK2 K17A/K18A LRRK2 Flag CMV Parkinson's Suzanne Pfeffer 186013 pCMV Flag-LRRK2...Cheryl Arrowsmith 213544 pCMV-mKO2-Parkin PRKN mKO2 CMV Parkinson's Lani Wu 213545 pCMV-Parkin(C431N)-A92mKO2...Parkinson's Lani Wu 213546 pCMV-Parkin(E16A) PRKN CMV Parkinson's Lani Wu 213547 pCMV-Parkin PRKN CMV Parkinson's...other Flag GST His TEV Other, non-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial...Spinocerebellar ataxia, infantile onset Smita Patel 205706 pCMV-alphaS-2 SNCA CMV Parkinson's Randy Schekman 205713...Parkinson's Lani Wu 213548 pCMV-Parkin-A92mKO2 PRKN mKO2 CMV Parkinson's Lani Wu 213549 pLv-CMV(trunc)-Parkin-A92mKO2...Dong-Er Zhang 13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1...
Showing: 1 - 14 of 14 results