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We narrowed to 11 results for: paav backbone

Showing: 1 - 11 of 11 results
  1. OCaMP: A New Calcium Indicator for Neural Imaging

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    Blog Post
    ... pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE pAAV Ef1a pAAV-hSyn-FLEX(cre)-OCaMP-WPRE pAAV hSyn ...Plasmid Backbone Promoter pRSET OCaMP pRSET T7 pAAV-hSYN1-NES-OCaMP-WPRE pAAV hSyn ... pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab has a limited quantity of AAVs that can be requested...
  2. Hot Plasmids: February 2026

    Type
    Blog Post
    ...#232922) pAAV2/5. Average + standard deviation from n = 2 (pAAV-hSyn-DIO- hM3D(Gq)-mCherry) or 3 (pAAV-hSyn-DIO-mCherry...increases AAV yield. Plasmids pAAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene #44361) and pAAV-hSyn-DIO-mCherry (Addgene... are arranged and regulated within the plasmid backbone.  Addgene designed and tested different configurations... produced dozens of AAV preps using these new backbones and consistently observe high yields across a ...and AAV5 packaging plasmids and found two (pAAV2/2 and pAAV2/5) that consistently produced higher yields...
  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...RNAs per gene. The lab modified the lentiviral backbone to encode the CD90.1 selection marker, Thy1.1,...from Yulong Li’s lab are now available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and 5HT sensors...
  4. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...domains The original iGluSnFR in the pMinDisplay backbone is fused to a specific peptide segment (PDGFR ... being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski...
  5. 27 Hot Plasmids from 2016

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    Blog Post
    ...Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-...levels using next gen sequencing. The STARR-seq backbone is available from Addgene in both human and fly...expression validated GPCR sequences to any desired backbone.  Kroeze, et al. Nat Struct Mol Biol. 2015. PubMed...Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila U6...biological applications. First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were...instance, the authors generated a GMAP-compatible backbone containing Rosa26 homology arms. The Rosa26 locus...a target gene in mice. The Jacks Lab used this backbone to target a CAG-driven loxP-stop-loxP cassette...
  6. Tetracycline Inducible Expression

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    Collection
    ...tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control...Browse the tables below to find popular empty backbones, expression vectors for transactivators, and vectors...expression of sgRNA TetR H1-2O2 Nathanael Gray 35625 pAAV-Ptet-RFP-shR-rtTA AAV Tet-On shRNA vector. To evaluate...inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber 60495 pSBtet-GP...Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled expression (On or Off) None...EF1a promoter Tet-On 3G rtTA David Vereide 120309 pAAV-FAH-rtTA3G AAV vector to express Tet-On 3G transactivator...promoter TetR Eric Campeau , Paul Kaufman 175274 pAAV-rtTA AAV Tet-On vector with neuron-specific expression...
  7. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange... present. Looking for more empty backbones? Visit our Empty Backbones page or search our complete collection...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...list many popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins,...A206K) pcDNA3.1-mGreenLantern - Mammalian Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern...Expression E2-Crimson 611 646 29 4.5 24 min Tetramer pAAV-CAG-E2-Crimson - Mammalian AAV Expression pEB2-E2...Monomer pcDNA-miRFP670nano3 - Mammalian Expression pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression...
  8. Allen Institute for Brain Science AAV Enhancer Collection

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    Collection
    ...230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m...214522 pAAV-AiE2402m-minBG-SYFP2-WPRE3-BGHpA AiP1706 AiE2402m SYFP2 Layer 5_ET Isocortex 223981 pAAV-AiE0464m-minBG-SYFP2...224006 pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA AiP12828 AiE0456m SYFP2 Layer 5_ET Isocortex 224016 pAAV-AiE0459m...224013 pAAV-AiE0674m-minBG-SYFP2-WPRE3-BGHp AiP12857 AiE0674m SYFP2 Layer 5_ET Isocortex 224017 pAAV-AiE0667m-minBG-SYFP2...214570 pAAV-AiE2575m-minBG-SYFP2-WPRE3-BGHpA AiP1888 AiE2575m SYFP2 Layer 6_CT Isocortex 230801 pAAV-AiE2575m-minBG-iCre...
  9. Zhang Lab CRISPR Page

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    Collection
    ...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and... SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons...guide acceptor: The PX552 plasmid (#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...
  10. Luciferase Plasmid Collection

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    Collection
    ...Luciferase blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which you ... Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs Other...Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α ...tet-inducible luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase...mays ubiquitin promoter. Paul Schulze-Lefert 83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase...pMKV060 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing firefly luc for monitoring plant transfection...pMKV057 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing WUS2 and IPT, with firefly luc for ...
  11. Fluorescent Protein Guide: In Vivo Imaging

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    Collection
    ...mNeptune2-N1 E2-Crimson 611 646 29 pEF.myc.ER-E2-Crimson pAAV-CAG-E2-Crimson Near-Infrared Protein Excitation ...Additional Resources Fluorescent Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization...
Showing: 1 - 11 of 11 results