...protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the...most cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing YGOI in...is your expression system or environment. The host organism will determine the type of vector that you...as close as possible to the basal expression level, induce expression at an opportune time or go all out...transforming wild-type yeast with an integrating vector expressing a selection cassette with 5′ and 3′ flanking...

...available in a mammalian expression vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius...APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and Cas9-mediated...ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry...both halves of the modified EB1 protein are overexpressed in cells, microtubule growth can be switched...

...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...Bacterial Expression pAquaN1 - Mammalian Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP -...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...pEiLOV-N1 - Mammalian Expression pGEX iLOV - Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian...Mammalian Expression mEmerald-pBAD - Bacterial Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ...

...His-tagged bacterial expression vector (see tagged expression vectors from the SGC... Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi...Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression pcDNA3 GFP LIC cloning ...destination vector for gene expression URA3 Yeast pXP518 - Yeast expression ... Gateway expression vector (see more Gateway vectors) TRP1 Yeast pXP216 - Yeast...Yeast expression vector (PGK1 promoter, 2μ) pXP316 - Yeast expression vector... Gateway expression vector (see more Gateway vectors) LEU2 Yeast pXP122 - ...

...protein expression constructs fused with epitopes or fluorophores, generate expression vectors with various...AAV transfer vector for in-vivo expression and plasmids optimized for dCas9-VPR expression in drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides ... HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA expressing cells were then ... to test multiple expression systems before they achieve the appropriate expression level for their gene...various promoters suitable for in vivo expression, and/or produce vectors to make mutant cell lines or transgenic... lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila U6:2 ...

...the lab used this vector in chick embryos, it also functions in mammalian expression systems.   Christopher...deposited mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core...with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate transcription. A lentiviral vector with... are an additional layer of control over gene expression that go beyond genomic sequence. Dysregulation...modifications are correlated with alterations in gene expression, but causality and mechanisms remain unclear....that help package DNA, were associated with gene expression or silencing. To alter DNA modifications, researchers...well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds H3K27ac marks...

...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...C-terminal Firefly luciferase fusion for expression in plants, co-expressed with mScarlet-I for ratiometric imaging...C-terminal Red Luciferase fusion for expression in plants, co-expressed with Gaussia luciferase for ratiometric...as a reference level of expression. Use this table to find plasmids expressing luciferase. We also offer... CMV Lentiviral expression of firefly luciferase Eric Campeau 18964 Luciferase-pcDNA3 Firefly CMV Mammalian...Retroviral expression of firefly luciferase Scott Lowe 14080 pAKlux2 Lux Operon Bacterial expression of luciferase...Renilla Actin5C Insect expression of renilla luciferase Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV...

...bacterial expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ... SFFV) or inducible (CMV-TO) cDNA expression, sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1...-miR30 (CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...enhancing target gene expression anywhere from 87x to 20,000x over endogenous expression (depending on the...

...present. By expressing Cre at specific times or locations, you can precisely control expression of your gene...developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter... or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the...visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher levels...Cre expression when cloning in E. coli . This alteration enables the generation of a single vector containing...Mombaerts 17408 Puro.Cre empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736...

...UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI...contains modified pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based ...Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing...much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs...placing TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...promoter, giving constitutive expression of TALORs built into this vector. 36034 pTAL6-BB Golden Gate TALEN...1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter N- and C-terminal tal...

...interest 85586 gRNA Cloning Vector Bbs I ver. 2 An empty sgRNA expression vector. sgRNA sequence can be cloned...plasmid, expresses 3xFNLDD in mammalian cells 48880 3xFNLDD/pMXs-I2 iChIP plasmid, expresses 3xFNLDD in...plasmid, expresses 3xFNLDD in mammalian cells 49536 3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD ...pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector...-neo Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector...plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter. 98041 Sa-dCas9-NLS-3xFLAG/pcDNA3.1 Expresses...Description 47948 3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis...

...plasmids, as depicted in the figure below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase...codon is often used. The gene of interest can be expressed from a plasmid containing a UAG codon at the place...with a different amino acid. They are typically expressed from a single plasmid, with multiple copies of...make sure that these conditions are true. Always express first with a control reporter gene – GFP for E....mCherry-GFP for mammalian cells. You should also express the protein with and without the ncAA in the media... tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI 31186 pEVOL-pAzF p-azidohenylalanine...mutated, serB deleted for phosphoserine GCE. For expressing phosphoserine-containing proteins using genetic...