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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11 results for: pcDNA3.1

Showing: 1 - 11 of 11 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a ...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Tuschl 26397 pF151 pcDNA3.1(+)SOD1WT SOD1 CMV ALS Elizabeth Fisher 26398 pF152 pcDNA3.1(+)SOD1A4V SOD1 CMV...Fisher 26399 pF153 pcDNA3.1(+)SOD1G37R SOD1 CMV ALS Elizabeth Fisher 26400 pF154 pcDNA3.1(+)SOD1G85R SOD1... Xu 29340 pcDNA3.1/GS-DJ1-R98Q-V5 PARK7 His, V5 CMV Parkinson's Mark Cookson 29341 pcDNA3.1/GS-DJ1-E18N-V5...Cookson 29342 pcDNA3.1/GS-DJ1-E18Q-V5 PARK7 V5 CMV Parkinson's Mark Cookson 29343 pcDNA3.1/GS-DJ1-L166P-V5...Cookson 29344 pcDNA3.1/GS-DJ1-E64D-V5 PARK7 His, V5 CMV Parkinson's Mark Cookson 29345 pcDNA3.1/GS-DJ1-D149A-V5...Cookson 29410 pcDNA3.1/GS-DJ1-C53A PARK7 His, V5 CMV Parkinson's Mark Cookson 29411 pcDNA3.1/GS-DJ1-A104T...Parkinson's Mark Cookson 29527 pcDNA3.1-ERBB4 ERBB4 CMV ALS Yardena Samuels 29528 pcDNA3.1-ERBB4 (E317K) ERBB4 CMV...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...LC3 mCherry, GFP Anne Brunet 40827 mCherry-hLC3B-pcDNA3.1 Autophagosome LC3 mCherry David Rubinsztein 11546...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression plasmid for cloning untagged proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells...Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian... including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian expression vector with N-terminal...plasmids with Neomycin selection Puromycin Mammalian pcDNA3.1-(-)-Pur - Eukaryotic expression vector Tet-pLKO-puro...

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...mGreenLantern 503 514 73 5.6 14 min Monomer (A206K) pcDNA3.1-mGreenLantern - Mammalian Expression pAAV-CAG-...hfYFP) 514 529 72 5.6 21 min Prone to dimerization pcDNA3.1-hfYFP - Mammalian Expression pBAD-hfYFP - Bacterial...Expression mhYFP 515 529 77 5.7 27 min Monomer pcDNA3.1-mhYFP - Mammalian Expression pBAD-mhYFP - Bacterial...Expression LSSmGFP 400 510 18 4.7 Monomer (A206K) pcDNA3.1-LSSmGFP - Mammalian Expression pBAD-LSSmGFP - ...Dendra2 490 553 507 573 405 23 19 6.6 6.9 Monomer pcDNA3.1 Dendra2 - Mammalian Expression pEG545 - Worm Expression...

  6. Luciferase Plasmid Collection

    Type
    Collection
    ...upstream of IRES-firefly luciferase. Bryan Welm 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...expression of firefly luciferase Javier Alcudia 113442 pcDNA3.1 NL NanoLuc® CMV Mammalian expression of Nanoluc...

  7. Fluorescent Proteins: FRET

    Type
    Collection
    ...610 72,000 0.22 5.3 1.9 mEGFP-N1 , mCherry2-N1 , pcDNA3.1 CMV mCherry-eGFP BGH Clover mRuby2 505 0.76 600...113,000 0.38 6.3 4.5 pcDNA3-Clover , pcDNA3-mRuby2 , pcDNA3.1-Clover-mRuby2 mClover3 mRuby3 506 0.78 592 128,000...

  8. TALEN Plasmids and Kits

    Type
    Collection
    ...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ..., pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla...

  10. Genetic Code Expansion

    Type
    Collection
    ...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182652 pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF) PylRS M. mazei...mazei Mammalian TAG Ivana Nikić-Spiegel 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...
Showing: 1 - 11 of 11 results