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  1. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Bob Weinberg pMD2.G VSV-G envelope...envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein for pseudotyping NanoMEDIC...applicable export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks...Akitsu Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and...all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing...this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging...determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus to infect many.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral... was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the...pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the same lab. Find pRSV-Rev. ...

  3. Lentivirus Plasmids

    Type
    Collection
    ... pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing...35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 17532 pLTR-G 2nd Envelope...generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for the FELIX (FIV-based) system...increases viral titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500...Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser...and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev,...VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, ...

  4. Retrovirus Plasmids

    Type
    Collection
    ...Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based...Salmon 8454 pCMV-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope...35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope ...Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab....pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For more... any nucleotide, R is a purine (A,G), and Y is a pyrimidine (C,U). G-C content should be 36-52%. Sense....3 Protocol for lentiviral infection and selection G. Safety H. References H.1 Published articles H.2 Web...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles. Note...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401 FuGENE...plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM TIP:...
Showing: 1 - 6 of 6 results