We narrowed to 9 results for: pcmv-myc

.... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...but was found to have a point mutation in the puromycin cassette that makes it less efficient in some ...with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd...PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient in some ...

...Gadella 231782 pCMV-mGold2t-CAF1-C-10 Nucleus CAF-1 mGold2t Francois St-Pierre 231774 pCMV-mGold2s-CAF1-...231780 pCMV-mGold2t-Actin-C-18 Actin filaments beta-Actin mGold2t Francois St-Pierre 231772 pCMV-mGold2s-Actin-C...231779 pCMV-mGold2t-Tubulin-C-18 Microtubules alpha-Tubulin mGold2t Francois St-Pierre 231771 pCMV-mGold2s-Tubulin-C...-h-Rab5a-c-Myc Endosomes (early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes (... 231777 pCMV-mGold2t-MannII-N-10 Golgi apparatus MannII mGold2t Francois St-Pierre 231769 pCMV-mGold2s-MannII-N...Imaging 231778 pCMV-mGold2t-Lysosome-N-20 Lysosomes Lamp1 mGold2t Francois St-Pierre 231770 pCMV-mGold2s-Lysosome-N...pTag-RFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Endosomes...

...target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian...of Venus-firefly luciferase Roland Friedel 170575 pCMV-FLuc Firefly CMV Retroviral expression of firefly...five transcriptional reporters for NF-kb, TGF-b, c-Myc, p53, and MAPK/JNK plus a constitutive control. Koen...FRB-Nluc : Split firefly luciferase reporter of rapamycin-inducible interaction. nLuc and cLuc : Constructs...

... transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic... activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow...are available at Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription...

...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For...kinase promoter drives expression of puromycin. Puro R Puromycin resistance gene for selection of pLKO...sulfate, and puromycin are located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration...differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin concentration...to fresh puromycin-containing media every other day. f. The minimum concentration of puromycin that results...with finer increments of puromycin to determine a more precise optimal puromycin concentration.) F.3. Protocol...

...system. Expresses gag-pol and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd Contains gag , pol , rev , tat , ...generation transfer plasmids. Didier Trono 12263 pCMV delta R8.2 2nd Contains gag , pol , rev , and tat...for the FELIX (FIV-based) system. Garry Nolan 8454 pCMV-VSV-G VSV-G-expressing envelope plasmid. Bob Weinberg...shRNA empty plasmid with puromycin selection. See plasmid 24150 for hygromycin selection. Bob Weinberg ...expression of shRNA with puromycin selection. See plasmid 21916 for neomycin selection. Dmitri Wiederschain...expression of shRNA with neomycin selection. See plasmid 21915 for puromycin selection. Dmitri Wiederschain...19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can be used for cDNA expression. David...

...13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's...Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep...LRRK2-WT LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted ... pGW1-Myc-DJ1-WT PARK7 V5 CMV Parkinson's Mark Cookson 29349 pGW1-Myc-DJ1-L166P/K130R PARK7 Myc CMV Parkinson's...D1994S)-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161585 pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc LRRK2...

...for the FELIX (FIV-based) system. Garry Nolan 8454 pCMV-VSV-G VSV-G envelope protein. Use with packaging...cells. See plasmid 1771 for puromycin selection, and plasmid 1774 for neomycin selection. Bob Weinberg 10676...expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A... For cloning and gene expression. Select with puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

... related pseudovirus assay. David Nemazee 170575 pCMV-FLuc Express firefly luciferase. Used in MLV-based...reporter vector expressing firefly luciferase with neomycin resistance. Christopher Vakoc 138152 pLentiEGFPdestablized...