.... Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab.... It can be used with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral..., this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral...but was found to have a point mutation in the puromycin cassette that makes it less efficient in some ...with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd...PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient in some ...

...158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C...pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Early endosomes...158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C...pTag-RFP-C-h-Rab11a-c-Myc Recycling endosomes Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-Myc Recycling...pTag-RFP-C-h-Rab4a-c-Myc Recycling endosomes Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc Recycling ...GFP Mitochondria ABCb10 GFP Orian Shirihai 158003 pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-...Mitochondria-Outer Membrane Mff acGFP1 Gia Voeltz 158007 pCMV-mGold-Mito-N-7 Mitochondria Cox8A mGold Francois...

...Expression System PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green...Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII) CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused...Cepko 13779 pRho-Cre Cre-Myc rhodopsin Mammalian Cepko 13780 pNrl-Cre Cre-Myc, Expressed in rod photorecetor...Simpson 106368 pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal...Hirrlinger 106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre... Green 8401 p224 pCMV-RFP/CREM floxed RFP within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin...ligand binding domain CMV Mammalian Green 12496 p270 pCMV-CRE-M-AR(LNCaP) CREM fused to mutant AR ligand binding...

... transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic... activity; protein purification vector  87439  pCMV-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow...are available at Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription...

...target sequence in the 3' UTR David Bartel 101139 pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian...of Venus-firefly luciferase Roland Friedel 170575 pCMV-FLuc Firefly CMV Retroviral expression of firefly...including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters Koen ...

...be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg’s lab...Note: pLKO.1 could also be packaged using pCMV-dR8.2 dvpr and pCMV-VSVG from the Robert Weinberg lab. For...kinase promoter drives expression of puromycin. Puro R Puromycin resistance gene for selection of pLKO...sulfate, and puromycin are located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration...differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin concentration...to fresh puromycin-containing media every other day. f. The minimum concentration of puromycin that results...with finer increments of puromycin to determine a more precise optimal puromycin concentration.) F.3. Protocol...

...plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd...and 3rd generation transfer plasmids Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev,...-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection...cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance...

...below: ID Plasmid Description 47948 3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian...iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD in mammalian...specific genomic regions of interest 63589 3xFN-Tel-TAL/pCMV-7.1 Expresses a 3xFLAG-tagged TAL protein recognizing...repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses...repeat. A retroviral expression vector with the neomycin resistance gene 64325 3xFLAG-dCas9/p-bacteria ...

...13313 pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's...Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep...LRRK2-WT LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted ... pGW1-Myc-DJ1-WT PARK7 V5 CMV Parkinson's Mark Cookson 29349 pGW1-Myc-DJ1-L166P/K130R PARK7 Myc CMV Parkinson's...D1994S)-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161585 pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc LRRK2...

...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...reporter vector expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab...

...pBS-CMV-gagpol Packaging MoMLV gag and pol Salmon 8454 pCMV-VSV-G Envelope Envelope protein; use with packaging... For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...