We narrowed to 4 results for: phelper

1. PEI Calculator for Planning AAV Packaging Transfections

   Type

   Blog Post

   ... 1 Transfer Plasmid : 1 pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type...

2. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   ...supplied on two different packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and VA RNA genes...

3. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   ...packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which ...

4. AAV Production in HEK293 Cells

   Type

   Protocol

   ... must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene of...μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer Plasmid 5,842 1.00 584.2...Volume Needed: 582.1 μg / 1.0 μg/μL = 582.1 μL pHelper: 0.08 μg/bp × 11,854 bp = 949.8 μg Volume Needed...