... 1 Transfer Plasmid : 1 pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type...

...products can be provided by helper plasmids (e.g., pHelper) and specific packaging cell lines (e.g., HEK293...

...packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which ...

...supplied on two different packaging plasmids: the pHelper plasmid containing Ad E2A, E4, and VA RNA genes...

... must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene of...μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer Plasmid 5,842 1.00 584.2...Volume Needed: 582.1 μg / 1.0 μg/μL = 582.1 μL pHelper: 0.08 μg/bp × 11,854 bp = 949.8 μg Volume Needed...