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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 27 results for: pina

Showing: 1 - 20 of 27 results

  1. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ... Other variants of Spinach include Baby Spinach, a miniaturized version of Spinach that retains similar... Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach. In doing so, they identified...was consequently brighter than Spinach. The initial studies using Spinach2 showed its ability to track RNA...imaging. Bunch of baby spinach, or BoBS, contains tandem repeats of Baby Spinach. It was developed in a...twice as bright as Spinach2 when bound to DFHBI. It’s also smaller than any form of Spinach - only 49 nucleotides...Addgene’s collection. Green fluorescent aptamers Spinach and Broccoli and their derivatives are aptamers..., but not quantitative, studies. Additionally, Spinach, the first aptamer identified to bind DFHBI, is...

  2. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...these devastating afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research...Bioresources in Naples, Italy. His group works on spinal muscular atrophy, a disease in which disruption...of the smn1 gene results in the degeneration of spinal cord motor neurons. This disease is estimated to...While disruption of the smn1 gene is known to cause spinal muscular atrophy, the molecular mechanisms behind...clinic and thereby restore motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal...Systematic review of incidence and prevalence of spinal muscular atrophy (SMA)." European Journal of Paediatric...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...first segments of the spinal cord showing the cortico spinal tract, CST, spinal cord and cranial nerve...Cuneate tract, dCST= dorsal cortico spinal tract, vCST= ventral cortico spinal tract, dc= decussation of the...cleared organs) method of imaging rodent brains and spinal cords by combining Ultramicroscopy with tetrahydrofuran-based...

  4. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...presence of that metabolite (Fig.2). Spinach eventually evolved into Spinach2, a more stable and brighter version...essentially mimicking GFP.  Though the aptamer, called Spinach, required longer exposure time than its protein...possibilities. For instance, further selection of Spinach with a target metabolite creates an aptamer that...also has a higher melting temperature than its Spinach ancestors, and has a lower magnesium dependence...Activity with GFP Resources at Addgene.org Find the Spinach Plasmids at Addgene Find Broccoli Plasmids at Addgene...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...bearing the "Bunch of Baby Spinach"aptamer, which is a brighter version of Spinach2, an RNA aptamer that fluoresces.... Bunch of Baby Spinach, or BoBS, consists of three tandem copies of the Baby Spinach core embedded in...with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your RNA experiments... - such as MS2 stem loops, PPL stem loops, the Spinach2 aptamer, and more - to sequences on a Gaussia ...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...MVD1A is effective in transducing brain cells and spinal cords of both C57BL/6J and BALB/cJ mice through...the brain, and a lower transduction rate in the spinal cord. Additionally, AAV.CAP-B10 is targeted away...used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic capsids for... neurons, with a lower transduction rate in the spinal cord. It is detargeted from the liver and shows...brain, except for the cerebellum, brain stem, and spinal cord. All PALs, including PAL1D, are detargeted...transduces a higher percentage of neurons in the spinal cord compared to AAV9 and AAV.MaCPNS2. Ex vivo ...

  7. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system. This created... tails still free, and different regions of the spinal cord were illuminated. By looking at groups of ...dissection of a behavioural module in the vertebrate spinal cord." Nature 461.7262 (2009): 407. PubMed PMID...

  8. Common Injection Routes in Mice

    Type
    Blog Post
    ...delivered to the central nervous system via cerebral spinal fluid. The injection site is the space in and around...minimally invasive. Restraint: anesthesia Location: spinal cord Gauge: 30 Volume: up to 0.01 ml (10 µl) Skill... 26 < 0.05 mL Advanced Intrathecal Anesthesia Spinal cord 30 up to 0.01 mL Expert Intratracheal Anesthesia...

  9. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Hideyuki Okano presented ground-breaking work on spinal cord injuries; Jessica Young shared research on..., a half-brother who is a quadriplegic due to a spinal cord injury, and numerous family friends who had...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...transduction in catecholamine pathways from the spinal cord (Wang, 2018, Metcalfe, 2022) and substantia...that are physically blocked, such as those in the spinal cord. Now that you understand this new tool, go...

  12. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...promoter results in robust transduction in the rat spinal cord following injury. Gene Therapy, 26(5), Article...adeno-associated viral vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...type IE Arthur Riggs 37057 GFP-SMN SMN1 GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1...Dementia Gerold Schmitt-Ulms 61946 4QQ6 SMN1 His T7 Spinal muscular atrophy Cheryl Arrowsmith 65106 pcDNA3... 71687 pcDNA3.1+ SMN1 myc HIS SMN1 His, Myc CMV Spinal muscular atrophy Gideon Dreyfuss 71875 pET-Sac-...Alzheimer's Michael Hecht 72286 pCI SMN1 SMN1 CMV Spinal muscular atrophy Elliot Androphy 72873 PMXS-SLC1A3...Alzheimer's Johanna Ivaska 156022 SMN1-1 SMN1 V5-MCP EF1a Spinal muscular atrophy Eugene Yeo 156073 TARDBP TARDBP...Shalem 215721 pDual-IN-SMN1 SMN1 GFP, tdTomato CAG Spinal muscular atrophy Chaolin Zhang 216225 TDP-43 mRuby...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...diseases such as hemophilia, muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and...
Showing: 1 - 20 of 27 results