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Showing: 1 - 7 of 7 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway destination vector for gene ...expression pLenti CMV/TO Zeo DEST - Tet-inducible lentiviral Gateway destination vector ...Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST - Lentiviral Gateway destination...Tet-inducible lentiviral shRNA expression pLenti CMV Hygro DEST - Lentiviral Gateway destination...expression through lentiviral delivery pLenti CMV GFP DEST - Lentiviral Gateway destination...destination vector for gene expression pLenti CMV/TO Hygro - Lentiviral Gateway... vector - Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA...

  2. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    .... For mammalian lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the... of Gateway cloning vectors Vector type  Vector features Purpose Donor vector attP sites for recombination...sequences into multiple vector systems. With the appropriate entry and destination vectors, one can use Gateway...ccdB gene is present in the donor vectors and the destination vectors prior to recombination, and it is...donor vector.   Method B: TOPO-cloning of the desired insert into an attL-entry-TOPO vector. TOPO...Gateway vector to produce the desired expression clone. This is possible thanks to the Gateway vectors’ design...generated entry clone and the attR sites of the destination vector. This reaction is catalyzed by the LR Clonase...

  3. Lentivirus Plasmids

    Type
    Collection
    ...for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...article for more variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase...Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian...Buck 98580 pLX304 Luciferase-V5 blast Firefly CMV Lentiviral expression of firefly luciferase with a V5 ...Marcel Bruchez 108542 pLenti-EF1a-Luciferase-IRES-Blast-WPRE Firefly EF1α Lentiviral expression of firefly...

  5. Cre-lox system

    Type
    Collection
    ...pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII...Gateway entry vector none Entry Vector Zon 27493 pBT140 Cre CMV Mammalian Luo 27546 pLM-CMV-R-Cre Cre and...empty vector Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 NLS-Cre CMV Mammalian...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton... zebrafish CMV Zebrafish Burgess 61570 Pvalb-2A-Cre targeting vector Cre; Targeting vector for Pvalb locus...copGFP and iCre CB Lentiviral Oka 72257 pCDH-CB-iCre iCre CB Lentiviral Oka 73350 pAdx-CMV-iCre-P2A-copGFP... iCre and GFP CMV Adenoviral Oka 73351 pAdx-CMV-iCre-P2A-tdTomato iCre and tdTomato CMV Adenoviral Oka...

  6. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter... reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter...below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter...reporter vector expressing firefly luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter...reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... DCTN1 CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina...G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-V5 KD PINK1 V5 CMV Parkinson's Mark...hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1 SLC1A3 CMV Episodic ataxia ... 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13318 pLenti6-DEST PINK1-V5 G309D...Black 23050 pDEST26-hOPTN OPTN His CMV ALS Jon Ashwell 23051 pDEST26-hOPTN-D474N OPTN His CMV ALS Jon Ashwell...Tuschl 26373 pDEST_TO_FUS FUS Flag, HA CMV ALS Thomas Tuschl 26374 pDEST_FUS FUS Flag, HA CMV ALS Thomas...PINK1 Myc CMV Parkinson's Mark Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson...
Showing: 1 - 7 of 7 results