...Retroviral shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway destination...expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLenti CMV Hygro DEST - ... Tet-inducible lentiviral shRNA expression pLenti CMV Neo DEST - Lentiviral Gateway ...expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pMKO.1 puro - Retroviral ...destination vector for gene expression pLenti CMV/TO Hygro - Lentiviral Gateway ...destination for shRNA expression pLenti CMV/TO Zeo DEST - Tet-inducible lentiviral...expression through lentiviral delivery pLenti CMV GFP DEST - Lentiviral Gateway ...

...variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro...plasmid 17618 for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive...Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid 24150...19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini ... See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression of mCherry...and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916...coexpression. Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression of RFP...

...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly...Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that...Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian...NanoLuc® CMV Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc....1-Luc Firefly CMV Mammalian expression of firefly luciferase Georg Weber 113450 pLenti NL NanoLuc® Ubc...Renilla CMV Mammalian expression of humanized renilla luciferase Witold Filipowicz 100984 pGL4.18 CMV-Luc ...of renilla luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of...

...lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible ...

...17446 pLenti CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter...library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and...library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...

...pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII...27493 pBT140 Cre CMV Mammalian Luo 27546 pLM-CMV-R-Cre Cre and mCherry coexpression CMV Lentiviral Sadelain...Lentiviral Oka 73350 pAdx-CMV-iCre-P2A-copGFP iCre and GFP CMV Adenoviral Oka 73351 pAdx-CMV-iCre-P2A-tdTomato...System PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401...CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding domain CMV Mammalian...split-Cre CMV Mammalian Hirrlinger 106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian ...probasin Mammalian Green 8406 p252 pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green...

... DCTN1 CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina...hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1 SLC1A3 CMV Episodic ataxia ...C-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13318 pLenti6-DEST PINK1-V5 G309D PINK1 V5 CMV Parkinson's Mark...-DJ1-A104T PARK7 CMV Parkinson's Mark Cookson 29412 pLenti6-DJ1-V5-E18N PARK7 V5 CMV Parkinson's Mark ... PARK7 His, V5 CMV Parkinson's Mark Cookson 29415 pLenti6-DJ1-V5-E18D PARK7 V5 CMV Parkinson's Mark Cookson...PARK7 V5 CMV Parkinson's Mark Cookson 29419 pLenti6-DJ1-V5-C53A PARK7 V5 CMV Parkinson's Mark Cookson 29527...moPrP(3F4) D177N PRNP CMV Dementia Susan Lindquist 1321 cytosolic moPrP (3F4) PRNP CMV Dementia Susan Lindquist...

...Plasmid Description Element On or off PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral reverse tetracycline-controlled... and fos 3'UTR tTA Off Mayford 38056 pXCX.CMV.tTA Adenoviral; CMV promoter; see article for neuronal-specific...cloning; selection cassette in format: PGK-rtTA-2A-puro; see article for tagged insert options rtTA On Root...promoter (Pbi); Pbi contains a TRE between two minimal CMV and is silent in absence of binding of tTA or rtTA...tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, and Blasticidin; can be used to make cell...generation rtTA On Campeau 25434 pMA2640 Retroviral; CMV-driven; linked via IRES to EGFP-Blasticidin fusion...

...Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base ...Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru 122871...Autophagosome LC3(ATG8) mCherry Maria Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru...158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C...158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C...GFP Mitochondria ABCb10 GFP Orian Shirihai 158003 pCMV-mGold-CAF1-C-10 Nucleus CAF-1 mGold Francois St-...Chromatin H2B mRFP Bryan Welm, Zena Werb 21045 pH2B_mCherry_IRES_puro2 Chromatin H2B mCherry Daniel Gerlich 25999...

...3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240 3xFLAG-dCas9/pMXs-puro Expresses...recognizing a telomere repeat 63590 3xFN-Tel-TAL/pMXs-puro Expresses a 3xFLAG-tagged TAL protein recognizing...below: ID Plasmid Description 47948 3xFLAG-dCas9/pCMV-7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian...iChIP plasmid, LexA-binding elements 48874 3xFNLDD/pCMV-7.1 iChIP plasmid, expresses 3xFNLDD in mammalian...specific genomic regions of interest 63589 3xFN-Tel-TAL/pCMV-7.1 Expresses a 3xFLAG-tagged TAL protein recognizing...repeat. A retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses...scaffold has efficient ver. 2 structure. 92220 pLenti_dCas9-2xAM Lentiviral plasmid expressing dCas9-2xAM...