...shRNA expression pLenti CMV Puro DEST - Lentiviral Gateway destination vector for gene ...Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLenti CMV Hygro DEST - ...shRNA expression pLenti CMV Neo DEST - Lentiviral Gateway destination vector for gene ...expression pLenti CMV/TO Zeo DEST - Tet-inducible lentiviral Gateway destination vector ... delivery pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene ...Lentiviral Gateway destination vector for gene expression pLenti CMV/TO Hygro - .../Bleo Mammalian pLenti X1 GFP-Zeo DEST - Lentiviral Gateway destination for shRNA ...

...for GFP plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or...variations. Campeau 39481 pLenti-puro 3rd CMV driven expression of cDNA. Puro selection. Shih 25737 pSLIK-Hygro...Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro resistance. See plasmid 24150...19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can be used for cDNA expression; puro resistance Sabatini ... See plasmid 21915 for Puro resistance. Wiederschain 36084 pLV-mCherry 3rd CMV driven expression of mCherry...and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916...coexpression. Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression of RFP...

...lentiviral expression, we could use a vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX...vector systems. With the appropriate entry and destination vectors, one can use Gateway to clone a gene ...generated entry clone and the attR sites of the destination vector. This reaction is catalyzed by the LR ...containing the ccdB gene is excised from the destination vector.  Once the BP and/or LR reactions are ...select the positive clones. The entry clone and destination vector carry different antibiotic resistance ...gene is present in the donor vectors and the destination vectors prior to recombination, and it is exchanged...expression clone, it is important to choose the destination vector that best fits your experiment. This choice...

...luciferase Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly...Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE : A splicing reporter that...Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian...NanoLuc® CMV Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc....1-Luc Firefly CMV Mammalian expression of firefly luciferase Georg Weber 113450 pLenti NL NanoLuc® Ubc...Renilla CMV Mammalian expression of humanized renilla luciferase Witold Filipowicz 100984 pGL4.18 CMV-Luc ...of renilla luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of...

...pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-CRE-M (BglII...27493 pBT140 Cre CMV Mammalian Luo 27546 pLM-CMV-R-Cre Cre and mCherry coexpression CMV Lentiviral Sadelain...Lentiviral Oka 73350 pAdx-CMV-iCre-P2A-copGFP iCre and GFP CMV Adenoviral Oka 73351 pAdx-CMV-iCre-P2A-tdTomato...System PI 8394 p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401...CREM CMV Mammalian Green 12494 p260 pCMV-CRE-M-AR(wt) CREM fused to AR ligand binding domain CMV Mammalian...split-Cre CMV Mammalian Hirrlinger 106370 pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian ...probasin Mammalian Green 8406 p252 pPr-CREM/CMV-STOP-luc CREM and CMV-STOP-luc cassette probasin Mammalian Green...

... DCTN1 CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina...G309D PINK1 V5 CMV Parkinson's Mark Cookson 13319 pLenti6-DEST PINK1-V5 KD PINK1 V5 CMV Parkinson's Mark...hATMS1981A ATM His, Flag CMV Ataxia telangiectasia Michael Kastan 32813 CMV-hEAAT1 SLC1A3 CMV Episodic ataxia ... 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13318 pLenti6-DEST PINK1-V5 G309D...Black 23050 pDEST26-hOPTN OPTN His CMV ALS Jon Ashwell 23051 pDEST26-hOPTN-D474N OPTN His CMV ALS Jon Ashwell...Tuschl 26373 pDEST_TO_FUS FUS Flag, HA CMV ALS Thomas Tuschl 26374 pDEST_FUS FUS Flag, HA CMV ALS Thomas...PINK1 Myc CMV Parkinson's Mark Cookson 13315 pcDNA-DEST53 PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson...

...Ciliary Base Chibby1 GFP Ken-Ichi Takemaru 89770 pLenti-EGFP-hChibby1 Mother Centrioles / Ciliary Base ...Base Chibby1 EGFP Ken-Ichi Takemaru 118084 pLenti-EB1-EGFP Microtubules EB1 EGFP Ken-Ichi Takemaru 122871...Autophagosome LC3(ATG8) mCherry Maria Harrison 38277 pMXs-puro GFP-p62 Autophagosome Sequestosome-1 EGFP Noboru...158008 pCMV-mGold-Tubulin-C-18 Microtubules alpha-tubulin mGold Francois St-Pierre 158009 pCMV-mGold-Actin-C...Robert Singer 27382 pDEST/N1-hEB1-GFP Microtubules EB1 GFP Robin Shaw 40908 pDEST/LIfeAct-mCherry-N1 Actin...Davidson 40907 pDEST/hCx43-EGFP-N1 Gap Junctions Connexin 43 EGFP Robin Shaw 49861 pDEST-mCherry-GJA1-20k-N1...158004 pCMV-mGold-Calnexin-N-14 Endoplasmic Reticulum Calnexin mGold Francois St-Pierre 158002 pCMV-mGold-Rab4a-C...