Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 23 results for: plex 307 plasmid

Showing: 1 - 20 of 23 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...biotinylating proteins with BirA. You can find plasmids that contain both AviTag and BirA at Addgene! ...discovered in the 1960s, strepavidin. The Biotin-avidin complex can then be used and/or manipulated for a scientist...immunoprecipitation experiments. Biotinylated proteins or complexes can be easily purified using columns or beads ... sample. Any free biotin-binding sites on this complex can bind to your biotinylated antibody. This creates...creates a large meshwork of biotin-avidin complexes bound to your protein of interest leading to amplification...This strategy is referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information.... PubMed PMID: 7479014. PubMed Central PMCID: PMC307283. 13. Howarth, M., & Ting, A. Y. “Imaging proteins...

  2. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...Blog Learn New Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR ...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...ligase." Nucleic acids research 24.15 (1996): 3071-3078. PubMed PMID: 8760896. PubMed Central PMCID: PMC146030...detection (14-20). LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...DNA ligase." Nucleic acids research 24.15 (1996): 3079-3085. PubMed PMID: 8760897. PubMed Central PMCID...Stefan V., and Göran Magnusson. "Sealing of gaps in duplex DNA by T4 DNA ligase." Nucleic acids research 10.5...

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...find all of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter...Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources at ...

  4. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...Allen Institute for Cell Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent... on the gene names in the figure below to find plasmids containing that particular gene. ... For subcellular markers in yeast see our plasmid collection from Sue Jaspersen (Stowers Institute...Prescott (Monash University). You can find more plasmids for labeling your subcellular structure of interest...interest in the Allen Institute for Cell Science Plasmid Collection. You can also use the library of full-length...Localization Poster here! References 1. Allen Institute Plasmid Pages 2. Cooper GM. 2000. The Cell: A Molecular...Resources on the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells Check...

  5. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each ligation...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...Background with Our CRISPR Guide Pages Find CRISPR Plasmids Find More Tools for Combinatorial Engineering ...was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder our attempts...simple tool that allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology was developed...of providing an easy-to-use tool to analyze the complex combinatorial genetic networks underlying your ...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. .... Today, it's time for another edition of Hot Plasmids!  Here's what you'll find in this post:  New...., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023)....therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D., et al. (2023). Cell type-specific...pulse-chase experiments, and more. Find LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID...LOV-Turbo into TransitID experiments.  Find TransitID plasmids here! These two developments open up new possibilities... promoter activity in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged...

  7. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...26422227. Find plasmids from this publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing...Francisella Cpf1 (FnCpf1), they employed an E. coli plasmid depletion assay to discover FnCpf1’s PAM sequence....1 (2016): 31-34. PubMed PMID: 27918548. Find plasmids from this publication at Addgene. 3. Kim, Daesik...Biotechnol. (2016). PubMed PMID: 27272384 Find plasmids from this publication at Addgene. Kleinstiver...Biotechnol. (2016). PubMed PMID: 27347757. Find plasmids from this publication at Addgene. Makarova, Kira...PMID: 26411297. Resources at Addgene Find CRISPR plasmids at Addgene Read Addgene's CRISPR Resource Guide...mediate cleavage rather than the multi-subunit complex employed by class 1 systems. A putative new class...

  8. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed cells yielded...Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin AA Jr (2016) Viral vectors...vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ... you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over...over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...stocks in a single plate with every plasmid or as individual plasmids shipped as agar stabs. Iverson, et...

  10. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...background via P1 transduction. Plasmid Expressing lambda red genes from a plasmid allows for a mobile recombineering... flexibility to modify the E. coli chromosome, plasmid DNA or BAC DNA.  To use the lambda red recombineering...bacteria with integrated defective prophage 2) from a plasmid, 3) from mini-λ or 4) from the lambda red phage...phage pL promoter.  It is a good idea to choose plasmids that also express the repressors associated with...limit leaky expression of the Red system.  Using a plasmid to express the lambda recombineering system is ... do this is to express lambda red genes from a plasmid with a heat sensitive origin of replication.  Once... them at 42℃.  Mini-λ A hybrid between using a plasmid and stable integration of a defective prophage ...

  11. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...percentage of surviving cells may be the result of plasmid integration via recombination during transfection...anything. Viruses are complicated and they perform complex biological tasks, both of which are factors in ...microbiology 37.6 (1999): 1683-1686. PubMed PMID: 10325307. PubMed Central PMCID: PMC84922. 5. Ugai, Hideyo.... PubMed PMID: 2339059. PubMed Central PMCID: PMC330758. Additional Resources on the Addgene Blog Learn...

  12. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ... CRISPR Resources on Addgene.org Find CRISPR plasmids and resources here Find pooled libraries available...activation." Cell 159.3 (2014): 647-661. PubMed PMID: 25307932. PubMed Central PMCID: MC4253859.  4. Konermann...transcriptional activation by an engineered CRISPR-Cas9 complex." Nature517.7536 (2015): 583. PubMed PMID: 25494202...imaging." Cell 159.3 (2014): 635-646. PubMed PMID: 25307933. PubMed Central PMCID: PMC4252608.  Additional...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Neurodegeneration Neurodegeneration Plasmids Neurodegeneration Plasmid Collection Addgene has a large collection...164210 pLEX-EF1a ANXA11-mEmerald ANXA11 mEmerald EF1a ALS Jennifer Lippincott-Schwartz 164211 pLEX-EF1a ...Browse all Neurodegeneration Plasmids Do you have suggestions for other plasmids that should be added to this...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...others. The table below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent...particular gene of interest, please search our full plasmid collection using the search bar at the top of this...

  14. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References Cullen, B. R. (...vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen, 1992) because of their ...beginning to realize that the situation is already complex enough that a deep understanding of retroviral ...Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiological Reviews, 56(3), 375...Viruses, 13(7), 1288. https://doi.org/10.3390/v13071288. PMID: 34372494. Prussin, A. J., Garcia, E. B....

  15. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... create fusions with a gene of interest. These plasmids will be most useful for constructing intermolecular...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...specific proteins in situ." FEBS journal 275.12 (2008): 3072-3087. PubMed PMID: 18479457. PubMed Central PMCID...

  16. Adenovirus Guide

    Type
    Guide
    ...you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene...shuttle plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements...for recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid (e.g. pAdTrack...backbone plasmid containing the adenoviral genes necessary for virus production. The shuttle plasmid and the...shuttle plasmid, but this method results in a higher background of non-recombinant adenoviral plasmids. Recombinant...

  17. CRISPR Guide

    Type
    Collection
    ...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...validated experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR...single plasmid ensures that all gRNAs will be expressed in the same cell. This is called multiplexing , and... the CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications...a single plasmid — though some have achieved targeting in the double digits. These multiplex gRNA vectors...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...specificity and activity are discovered. Browse Plasmids: Single-Strand Break (Nick) , Double-Strand Break...

  18. Immunology Research Plasmids and Resources

    Type
    Collection
    ... Plasmid Collections Immunology Research Immunology Research Plasmids and Resources You...Fluorescent Proteins Cancer Research Plasmids Gene Categories Plasmid Tables Additional Resources Mammalian...to the available plasmids for each gene. Please note that not all genes have plasmids available for request...and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for each gene...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...rapidly initiate a secondary immune response. Browse Plasmids for Immunology Research Genes have been grouped...

  19. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...posts , or find CRISPR plasmids for your research. Addgene has CRISPR plasmids for the following models... in the field, and enabling access to multiple plasmids used for various platform applications. For more...nuclease form a complex with each individual, unique crRNA. (4) Each crRNA:tracrRNA:Cas9 complex seeks out ...genome engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR...a protospacer adjacent motif (PAM). The CRISPR complex binds to the foreign DNA and cleaves it to destroy...after where the crRNA would bind. (5) After the complex binds, Cas9 separates the double stranded DNA target...strands near the PAM. (6) The crRNA:tracrRNA:Cas9 complex unbinds after the double-strand break. CRISPR systems...

  20. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid that does...gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids By Function Genome ... these independent gRNA plasmids can be paired with a wide variety of plasmids and therefore are not limited.... Whether the plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that...none, need plasmid 65775 N. meningitidis Joung VVT1 65779 Mammalian BsmBI none, need plasmid 65776 S. aureus...expression of Csy4 (eg Plasmid 53369 ). Joung pX333 Mammalian Single all-in-one plasmid for tandem expression...assembly method: plasmids for assembly of single gRNA vectors with various promoters, plasmids for assembling...
Showing: 1 - 20 of 23 results