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We narrowed to 3 results for: plko 1 scramble shrna

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  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...lentiviral vector that contains a scrambled shRNA. This scrambled shRNA is used as a negative control for...mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector ...standard plasmids. Find pLKO.1 - TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary...and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1 protocol. Pro-tip: ...expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini’s lab is...particles. It’s capable of inducible expression of shRNA in target cells by the addition of tetracycline/...target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its name ...

  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with...item of 2005. shRNA - In 2006, we saw an increase in popularity of empty backbones for shRNA expression,...beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty backbones...with the pLKO plasmids you'll note that those are lentiviral constructs. In 2007 we continued to see requests...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...non-hairpin insert. pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging...Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2 Annealing...encoded in pLKO.1 allows for convenient stable selection. Figure 1 : Map of pLKO.1 containing an shRNA insert...website and “search for “pLKO”“. Back to Top B. Designing shRNA Oligos for pLKO.1 B.1 Determining the Optimal...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...information Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning...
Showing: 1 - 3 of 3 results