... envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus... gets a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the...effects. To date, nearly 900 articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets... pmd2.g...times (over 800 orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful...determine the types of cells that it can infect. The VSV-G envelope is widely used and requested because it enables...

... all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing...pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited by the Bob Weinberg lab and contains...s meant to be used with pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the...pRSV-Rev and the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from ...plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from... which types of cells a virus can infect. The VSV-G envelope protein enables this virus to infect many...plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging...

...Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope ...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...Akitsu Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information...applicable export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks...

...12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing...pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 17532 pLTR-G 2nd Envelope Expression... plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for the FELIX (FIV-based) system Nolan...viral titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500 pNHP 2nd...Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser 17531...deficient lentivirus Rawlings , Scharenberg 12251 pMDLg/pRRE 3rd Packaging Contains Gag and Pol; can be ...

...-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope VSV-G-expressing...envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based) system Nolan...

...lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids from Didier...

...  Fig. 1: The monomer of Plasmid 12259: pMD2.G (depicted on the left) is ~5.8 kb in length. The...Resources and References References Bacolla A, Wang G, Jain A, Chuzhanova NA, Cer RZ, Collins JR, Cooper...

... 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab. Cell...

... 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell...

...Interchangeable: usually encodes for VSV-G Interchangeable: usually encodes for VSV-G Safety Safe. Replication incompetent...expression. The envelope protein Env (usually VSV‐G due to its wide infectivity) is encoded on a third... with receptors at the host cell surface. The VSV-G envelope protein is commonly used in lentiviral particle...Envelope VSVG in trans Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein used ...packaging plasmid and rev from another, such as pMDLg/pRRE and pRSV-Rev . 3rd generation packaging systems...

... any nucleotide, R is a purine (A,G), and Y is a pyrimidine (C,U). G-C content should be 36-52%. Sense...Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral particles....Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401 ... plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-free OPTI-MEM TIP....3 Protocol for lentiviral infection and selection G. Safety H. References H.1 Published articles H.2 Web.... Incubate for 20-30 minutes at room temperature. g. Retrieve HEK-293T cells from incubator. The cells...positive control for the puromycin selection. Days 4+: g. Change to fresh puromycin-containing media as needed...

... Plasmid ( psPAX2 ) 1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro...