Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 7 results for: pmd2 g envelop plasmid

Showing: 1 - 7 of 7 results

  1. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab...lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders... a sense of the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the secretome...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...the VSV-G envelope protein under the control of the powerful mammalian CMV promoter. Envelope proteins...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors...the types of cells that it can infect. The VSV-G envelope is widely used and requested because it enables...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid. Envelope...and an envelope expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find...pRSV-Rev and the envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David...of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (...used with pMDLg/pRRE and the envelope expressing plasmid pMD2.G, both deposited from the same lab. Find...available plasmid in the top 15. Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited... top requested plasmid from 2016. You may notice the “V2.0” at the end of this plasmid which indicates...

  3. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein... and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope protein for...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster,...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...

  4. Retrovirus Plasmids

    Type
    Collection
    ...-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 22501 pHEF-VSVG Envelope VSV-G-expressing...γ-Retrovirus Envelope and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid...VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based) system...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...system Nolan 35617 pCAG-Eco Envelope Ecotropic MLV expressing envelope plasmid Nienhuis and Salmon 35616...VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer...

  5. Lentivirus Plasmids

    Type
    Collection
    ...generation transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G...pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis and Salmon 17532 pLTR-G 2nd Envelope Expression...pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging ...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components... Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500 pNHP 2nd Packaging... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid to 20 μl serum-...shRNA. psPAX2 Packaging plasmid for producing viral particles. pMD2.G Envelope plasmid for producing viral...to vary the ratio of shRNA plasmid, packaging plasmid, and envelope plasmid to obtain the ratio that gives... also be used with packaging plasmid pCMV-dR8.2 dvpr and envelope plasmid pCMV-VSVG from Robert Weinberg...Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells GenHunter: #Q401 ... any nucleotide, R is a purine (A,G), and Y is a pyrimidine (C,U). G-C content should be 36-52%. Sense....1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol Version 1.0. December 2006. Copyright...

  7. Lentivirus Production

    Type
    Protocol
    ...1.3 pmol Envelope Plasmid ( pMD2.G ) 0.72 pmol Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume...DNAs: Lentiviral Packaging plasmid(s), Envelope plasmid, and Transfer plasmid encoding your insert of interest...transfection plasmids into OptiPro SFM: Reagent Amount per 10 cm dish* Packaging Plasmid ( psPAX2 ) 1.3...volume 500 μL *Plasmid concentrations and ratios should be optimized for each transfer plasmid. Pro-Tip Endotoxins...therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, ... cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be..., the plasmids should also be propagated in an endonuclease negative E. coli strain such as NEB stable...
Showing: 1 - 7 of 7 results