...predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation lentiviral packaging plasmid...pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral...contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2.G. Find...Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation...plasmid from the Trono lab contains the rev gene. It’s meant to be used with pMDLg/pRRE and the envelope...contains all the necessary elements for packaging, reverse transcription, and integration needed for the production...

...Generation Type Description PI 12253 pRSV-Rev 3rd Packaging Contains Rev; can be used with 3rd generation ...with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used...pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used with 2nd and 3rd ...22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part of the ...the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N/A Envelope Ecotropic MLV expressing...pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. Use with envelope plasmids from Jakob Reiser. Reiser...

...one packaging plasmid and rev from another, such as pMDLg/pRRE and pRSV-Rev . 3rd generation packaging...plasmid: Gag, Pol, Rev, Tat Two plasmids: one encoding Gag and Pol and another encoding Rev Envelope Plasmid...by Nucleocapsid. RRE in cis Rev Response Element; sequence to which the Rev protein binds. WPRE in cis ...Precursor protein containing Reverse Transcriptase and Integrase components. Rev in trans On separate plasmid... single packaging plasmid encoding the Gag, Pol, Rev, and Tat genes. The transfer plasmid contains the... system is split into two plasmids: one encoding Rev and one encoding Gag and Pol. Although safer, this...generation packaging systems: express the HIV gag, pol, rev, and tat genes all from a single packaging plasmid...