...from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd...The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...plasmid from the Trono lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope...most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

...Contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev ...Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/Pol, Tat, Rev. Use ...Trono 12263 pCMV delta R8.2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be used with 2nd and 3rd generation...Weinberg 8455 pCMV-dR8.2 dvpr 2nd Packaging Contains Gag, Pol, Rev, Tat, Vif, and Nef; can be used with 2nd ...plasmid Kasparov 22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging...Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N/A Envelope Ecotropic...

...contains Gag, Pol, Rev, and Tat genes. Specifically for our knowledge here, the Pol gene encodes three separate... an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal...lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G...were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt et ...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single..., Berry, C., Ecker, J. R., & Bushman, F. (2002). HIV-1 integration in the human genome favors active genes...19491821 PubMed Central PMCID: PMC2835240 Wu Y. (2004). HIV-1 gene expression: lessons from provirus and non-...

...one plasmid: Gag, Pol, Rev, Tat Two plasmids: one encoding Gag and Pol and another encoding Rev Envelope... split into two plasmids: one encoding Rev and one encoding Gag and Pol. Although safer, this system can...express the HIV gag, pol, rev, and tat genes all from a single packaging plasmid such as psPAX2 . 3rd generation...system contains a single packaging plasmid encoding the Gag, Pol, Rev, and Tat genes. The transfer plasmid... Interchangeable: usually encodes for VSV-G Interchangeable: usually encodes for VSV-G Safety Safe. Replication...Replication incompetent: Uses 3 separate plasmids encoding various HIV genes. Safer. Replication incompetent and...generation packaging systems: express gag and pol from one packaging plasmid and rev from another, such...

...Once introduced, the puromycin resistance marker encoded in pLKO.1 allows for convenient stable selection...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles...an environment (e.g. BL2+) suitable for handling HIV-derivative viruses. For transient knockdown of protein...Recommended Materials Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells... transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid...μg/mL polybrene. TIP: Polybrene increases the efficiency of viral infection. However, polybrene is toxic...drives RNA Polymerase III transcription for generation of shRNA transcripts. cPPT Central polypurine tract,...