We narrowed to 4 results for: pspax2 encoding hiv gag pol

...from the Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd...The top 15 plasmids requested from Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid...plasmid from the Trono lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope...most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also from the Didier Trono lab is pMD2...2A-Puro (PX459) - This mammalian expression vector encodes both SpCas9 and your gRNA. It’s the former version...

...system. Expresses gag-pol and rev . Garry Nolan 8455 pCMV-dR8.2 dvpr 2nd Contains gag , pol , rev , tat , ...Contains gag and pol . Can be used with 3rd generation transfer plasmids along with a plasmid encoding rev ...packaging components. Didier Trono 12260 psPAX2 2nd Contains gag , pol , rev , and tat . Can be used with 2nd...Didier Trono 12263 pCMV delta R8.2 2nd Contains gag , pol , rev , and tat . Can be used with 2nd and 3rd... Didier Trono 17531 pCD/NL-BH*DDD 2nd Contains gag-pol , tat , and rev . Use with envelope plasmids from...titer. Sergey Kasparov 22500 pNHP 2nd Contains gag , pol , rev , and tat . Sergey Kasparov Return to top...Tobias Meyer 86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin...

...contains Gag, Pol, Rev, and Tat genes. Specifically for our knowledge here, the Pol gene encodes three separate... an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal...lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G...were first discovered by researchers investigating HIV integrase mutants in the early 1990s (Leavitt et ...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single..., Berry, C., Ecker, J. R., & Bushman, F. (2002). HIV-1 integration in the human genome favors active genes...19491821 PubMed Central PMCID: PMC2835240 Wu Y. (2004). HIV-1 gene expression: lessons from provirus and non-...

...Once introduced, the puromycin resistance marker encoded in pLKO.1 allows for convenient stable selection...Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid for producing viral particles...an environment (e.g. BL2+) suitable for handling HIV-derivative viruses. For transient knockdown of protein...Recommended Materials Material Vendor and catalog # psPAX2 Addgene #12260 pMD2.G Addgene #12259 HEK-293T cells... transfection: 1 μg pLKO.1 shRNA plasmid 750 ng psPAX2 packaging plasmid 250 ng pMD2.G envelope plasmid...μg/mL polybrene. TIP: Polybrene increases the efficiency of viral infection. However, polybrene is toxic...drives RNA Polymerase III transcription for generation of shRNA transcripts. cPPT Central polypurine tract,...