...by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

...11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display collateral...Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell, 163(3): 759-771 (2015). 10.1016...Cas9’. The Cas9 nuclease is a genome engineering powerhouse, and for good reason. It is guided by an RNA ...

...also successfully regulated expression of channelrhodopsin-2, commonly used in optogenetics, opening up... Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific manipulation...

...retroviral library was used to transduce T helper type 2 (Th2) cells from mice that constitutively express ...Gottschalk lab has surveyed a range of microbial rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis...membranes, which might beneficially influence the rhodopsin properties. Find these voltage biosensors at Addgene...

... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...fluorescent labels. In Nature Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038...and mitophagy, can be tracked using dyes like Rhodamine 123 (R123) (Liu et al., 2017). In multi-color ...diffraction limit of light. Dyes like silicon-rhodamine or Janelia Fluor (JF) dyes have specific properties...

...immediate early gene expression. Genes Dev. 1988 Jun;2(6):718-29. doi: 10.1101/gad.2.6.718. PMID: 2843425...Targeted Gene Expression in Drosophila.” Genetics 186(2) (2010): 735-55. PubMed PMID: 20697123. PubMed Central... in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197-214. PubMed PMID: 22129153. Additional...//doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Cumate-inducible gene expression...

...with neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church...Patrick Miller-Rhodes from the University of Rochester Medical Center. Patrick Miller-Rhodes is currently...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...

...light sensing proteins. Furthermore, during the first 2 weeks of life, larvae already exhibit distinct behaviors..."Optical control of zebrafish behavior with halorhodopsin." Proceedings of the National Academy of Sciences...

...articles, such as: Sander JD & Joung JK, Nature Biotech, 2 March 2014. Or browse the articles related to the ...be inserted into the genome using CRISPR/Cas forHomologous Recombination (HR)? How long should the homology...

...another. You’ll need to validate that points 1 and 2 hold true under your specific experimental conditions...when (1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets...your bioinformatics skills (or your friendly neighborhood bioinformatician) to look at RNA expression ...

...inside the cell,” says Pedre Pérez. Figure 2: Micrographs of the biosensor expressed in the human...method allowed Tian to optimize a red‑shifted archaerhodopsin‑derived genetically encoded voltage indicator...

...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV...

...dimerization-dependent protein interactions. FEBS letters, 591 2, 415-424. PubMed PMID: 28032891. This is a different...and BioID, any protein that’s within a ~10nm neighborhood of the bait protein will be biotinylated. This...

...PubMed PMID: 9618448. PubMed Central PMCID: PMC98921. 2. Rakowski, Sheryl A., and Marcin Filutowicz. "Plasmid.... PubMed Central PMCID: PMC390392. 4. Feinbaum, Rhonda. "Introduction to plasmid biology." Current Protocols...

...work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes...Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work/life balance. Is it truly possible to...